Phillips M A, Stewart B E, Qin Q, Chakravarty R, Floyd E E, Jetten A M, Rice R H
Department of Environmental Toxicology, University of California, Davis 95616.
Proc Natl Acad Sci U S A. 1990 Dec;87(23):9333-7. doi: 10.1073/pnas.87.23.9333.
The nucleotide and deduced amino acid sequences of the coding regions of human and rat keratinocyte transglutaminases (protein-glutamine: amine gamma-glutamyltransferase; EC 2.3.2.13) have been determined. These yield proteins of approximately 90 kDa that are 92% identical, indicative of the conservation of important structural features. Alignments of amino acid sequences show substantial similarity among the keratinocyte transglutaminase, human clotting factor XIII catalytic subunit, guinea pig liver tissue transglutaminase, and the human erythrocyte band-4.2 protein. The keratinocyte enzyme is most similar to factor XIII, whereas the band-4.2 protein is most similar to the tissue transglutaminase. A salient feature of the keratinocyte transglutaminase is its 105-residue extension beyond the N terminus of the tissue transglutaminase. This extension and the unrelated activation peptide of factor XIII (a 37-residue extension) appear to be added for specialized functions after divergence of the tissue transglutaminase from their common lineage.
已确定人及大鼠角质形成细胞转谷氨酰胺酶（蛋白质 - 谷氨酰胺：胺γ - 谷氨酰转移酶；EC 2.3.2.13）编码区的核苷酸序列及推导的氨基酸序列。这些序列产生约90 kDa的蛋白质，它们的同源性为92%，这表明重要结构特征具有保守性。氨基酸序列比对显示，角质形成细胞转谷氨酰胺酶、人凝血因子XIII催化亚基、豚鼠肝组织转谷氨酰胺酶和人红细胞带4.2蛋白之间存在显著相似性。角质形成细胞酶与因子XIII最为相似，而带4.2蛋白与组织转谷氨酰胺酶最为相似。角质形成细胞转谷氨酰胺酶的一个显著特征是其在组织转谷氨酰胺酶N端之外有105个残基的延伸。在组织转谷氨酰胺酶从其共同谱系分化后，这个延伸部分以及因子XIII不相关的激活肽（37个残基的延伸）似乎是为了特殊功能而添加的。
