Tucker K D, Wilkins T D
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Infect Immun. 1991 Jan;59(1):73-8. doi: 10.1128/iai.59.1.73-78.1991.
Clostridium difficile causes pseudomembranous colitis in humans. The enterotoxin (i.e., toxin A) from this organism is believed to be responsible for the initial intestinal pathology associated with this disease. Previous work shows that this toxin binds to carbohydrates that contain Gal alpha 1-3Gal beta 1-4GlcNAc. However, this carbohydrate is not present on normal human cells. Therefore, this study was undertaken to identify potential receptors for toxin A that do exist on human intestinal epithelium. Using enzyme-linked immunosorbent assay, affinity chromatography, and altered migration in an electric field, we assayed the binding of toxin A to purified carbohydrates and glycoproteins. We found that toxin A bound to the carbohydrate antigens designated I, X, and Y. Each of these carbohydrates exist on the intestinal epithelium of humans.
艰难梭菌可导致人类发生伪膜性结肠炎。该病原体产生的肠毒素（即毒素A）被认为是引发与该疾病相关的初始肠道病变的原因。先前的研究表明，这种毒素可与含有Galα1-3Galβ1-4GlcNAc的碳水化合物结合。然而，这种碳水化合物在正常人体细胞中并不存在。因此，开展本研究以鉴定人类肠上皮细胞上确实存在的毒素A潜在受体。我们利用酶联免疫吸附测定、亲和层析以及电场中迁移变化，检测了毒素A与纯化的碳水化合物和糖蛋白的结合情况。我们发现毒素A可与命名为I、X和Y的碳水化合物抗原结合。这些碳水化合物均存在于人类的肠上皮细胞中。
