McKibbin J M, Spencer W A, Smith E L, Mansson J E, Karlsson K A, Samuelsson B E, Li Y T, Li S C
J Biol Chem. 1982 Jan 25;257(2):755-60.
Glycolipids containing fucose linked to N-acetylglucosamine were isolated and characterized from 14 individual human and 13 individual dog intestines. From 8 of the dog intestines, Lewis a isomer fucolipids were isolated, all identical and having the structure Gal(beta 1 leads to 4)[Fuc alpha 1 leads to 3]GlcNAc(beta 1 leads to 3)Gal(beta 1 leads to 4)Glc-ceramide. Lewis b isomer fucolipids were isolated from 12 of the intestines, all identical and having the structure Fuc(alpha 1 leads to 2)Gal(beta 1 leads to 4)[Fuc alpha 1 leads to 3]GlcNAc(beta 1 leads to 3)Gal(beta 1 leads to 4)Glc-ceramide. Lewis a-active glycolipids were isolated as the sole major fucolipid in 6 of the human intestines and differed from the canine isomer only in the position of the linkage of galactose to N-acetylglucosamine, having the beta 1 leads to 3 (type 1) rather than the beta 1 leads to 4 (type 2) linkage. Lewis b-active fucolipids were isolated from 8 human intestines and differed from their canine isomer only in that they, too, had the type 1 rather than the type 2 oligosaccharide chain. Lewis a and b glycolipid isomers commonly co-existed in canine intestine as major fucolipids whereas Lewis a and b glycolipids did not so co-exist in human intestine. In all of the fucolipids, only hydroxylated fatty acids were present and phytosphingosine and sphingosine were the predominant long chain bases. These findings are of interest in the biosynthesis of these substances and in their genetic expression.
从14例人肠道和13例犬肠道中分离并鉴定了含有与N - 乙酰葡糖胺相连的岩藻糖的糖脂。从8例犬肠道中分离出Lewis a异构体岩藻糖脂，它们均相同，结构为Gal(β1→4)[Fucα1→3]GlcNAc(β1→3)Gal(β1→4)Glc - 神经酰胺。从12例肠道中分离出Lewis b异构体岩藻糖脂，它们也均相同，结构为Fuc(α1→2)Gal(β1→4)[Fucα1→3]GlcNAc(β1→3)Gal(β1→4)Glc - 神经酰胺。Lewis a活性糖脂在6例人肠道中作为唯一主要的岩藻糖脂被分离出来，它与犬异构体的区别仅在于半乳糖与N - 乙酰葡糖胺的连接位置，其为β1→3（1型）而非β1→4（2型）连接。从8例人肠道中分离出Lewis b活性岩藻糖脂，它与其犬异构体的区别仅在于它也具有1型而非2型寡糖链。Lewis a和b糖脂异构体在犬肠道中通常作为主要岩藻糖脂共存，而Lewis a和b糖脂在人肠道中并非如此共存。在所有岩藻糖脂中，仅存在羟基化脂肪酸，植物鞘氨醇和鞘氨醇是主要的长链碱。这些发现对于这些物质的生物合成及其基因表达具有重要意义。
