Surcel H M, Tapaninaho S, Herva E
National Public Health Institute, Oulu, Finland.
Clin Exp Immunol. 1991 Jan;83(1):112-5. doi: 10.1111/j.1365-2249.1991.tb05598.x.
The specific cell-mediated cytotoxicity of tularaemia-immune human T lymphocytes were studied in vitro. Peripheral blood mononuclear cells (PBMC) of six tularaemia-vaccinated healthy subjects were stimulated with F. tularensis LVS whole cell antigen for 6 days and used as effector cells in a conventional 4-h 51Cr release cytotoxicity assay. The target cells were phagocyting autologous monocytes, which were pulsed with F. tularensis or PPD antigen. The specific lysis of the F. tularensis-pulsed cells (42.6% +/- 11.7) was significantly higher (P less than 0.05) than that of the PPD-pulsed ones (22.2% +/- 8.3) or unpulsed control cells (15.9% +/- 5.2). The cytotoxicity was associated with CD4+ F. tularensis-specific T cell clones (TLC), which killed 36.3% +/- 12.3 of the F. tularensis-pulsed targets but only 6.9% +/- 6.5 of the unpulsed control targets. Their lysing was inhibited by monoclonal anti-HLA-DR and anti-HLA-DQ antibodies, but not by CD15 (monocyte/macrophage) antibody. The functional role of CD4+ lymphocytes in tularaemia immunity is discussed.
对兔热病免疫的人T淋巴细胞的特异性细胞介导细胞毒性进行了体外研究。用土拉弗朗西斯菌LVS全细胞抗原刺激6名接种兔热病疫苗的健康受试者的外周血单核细胞（PBMC）6天，并将其用作传统4小时51Cr释放细胞毒性试验中的效应细胞。靶细胞是吞噬了自体单核细胞的细胞，这些单核细胞用土拉弗朗西斯菌或结核菌素纯蛋白衍生物（PPD）抗原进行脉冲处理。经土拉弗朗西斯菌脉冲处理的细胞的特异性裂解率（42.6%±11.7）显著高于经PPD脉冲处理的细胞（22.2%±8.3）或未脉冲处理的对照细胞（15.9%±5.2）（P<0.05）。细胞毒性与CD4+土拉弗朗西斯菌特异性T细胞克隆（TLC）有关，该克隆可杀死36.3%±12.3的经土拉弗朗西斯菌脉冲处理的靶细胞，但只能杀死6.9%±6.5的未脉冲处理的对照靶细胞。它们的裂解受到单克隆抗HLA-DR和抗HLA-DQ抗体的抑制，但不受CD15（单核细胞/巨噬细胞）抗体的抑制。文中讨论了CD4+淋巴细胞在兔热病免疫中的功能作用。
