Strockbine N A, Parsonnet J, Greene K, Kiehlbauch J A, Wachsmuth I K
Enteric Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Infect Dis. 1991 Feb;163(2):406-9. doi: 10.1093/infdis/163.2.406.
During 1988 the number of Shigella dysenteriae type 1 infections reported in the United States increased fivefold. To determine if recent isolates from Mexico were related to those that caused epidemics of dysentery worldwide, Southern hybridization analysis was done with Shiga toxin and ribosomal RNA gene probes. Western hemisphere and Eastern Hemisphere strains differed by the size of a single EcoRI fragment carrying the Shiga toxin genes. Three ribosomal DNA (rDNA) patterns were observed, which correlated with the strain's continental origin for 81 of 83 isolates tested. Together the Shiga toxin and rDNA probe results indicated that recent Mexican isolates were chromosomally similar to earlier Central American isolates and distinct from Asian and African strains. This suggests there has been no significant exchange of organisms between continents in recent decades and that the 1988 outbreak in Mexico was caused by strains present in Central America since at least 1962.
1988年期间，美国报告的1型志贺氏痢疾杆菌感染病例数量增加了五倍。为了确定近期从墨西哥分离出的菌株是否与在全球范围内引发痢疾流行的菌株有关，使用志贺毒素和核糖体RNA基因探针进行了Southern杂交分析。西半球和东半球的菌株在携带志贺毒素基因的单个EcoRI片段大小上存在差异。观察到三种核糖体DNA（rDNA）模式，在测试的83株菌株中，有81株的模式与菌株的大陆来源相关。志贺毒素和rDNA探针结果共同表明，近期从墨西哥分离出的菌株在染色体上与早期从中美洲分离出的菌株相似，与亚洲和非洲菌株不同。这表明近几十年来各大洲之间没有发生显著的生物体交换，并且1988年墨西哥的疫情是由至少自1962年以来就存在于中美洲的菌株引起的。
