一种从枸橼酸化全血中分离基因组DNA的快速方法。
A rapid method for the isolation of genomic DNA from citrated whole blood.
作者信息
Parzer S, Mannhalter C
机构信息
Department of Medicine, University of Vienna, Austria.
出版信息
Biochem J. 1991 Jan 1;273(Pt 1)(Pt 1):229-31. doi: 10.1042/bj2730229.
Abstract
We have developed a DNA isolation method which allows the isolation of high quality DNA from frozen citrated blood and cell lines within 90 min. The procedure avoids the use of toxic organic reagents and tedious extraction steps. DNA prepared by this method is very well suited for restriction fragment length polymorphism and polymerase chain reaction analysis.
摘要
我们已经开发出一种DNA分离方法，该方法能够在90分钟内从冷冻的枸橼酸盐抗凝血和细胞系中分离出高质量的DNA。该程序避免使用有毒的有机试剂和繁琐的提取步骤。用这种方法制备的DNA非常适合用于限制性片段长度多态性分析和聚合酶链反应分析。
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