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用于限制性片段长度多态性分析的快速DNA纯化

Rapid DNA purification for restriction fragment length polymorphism analysis.

作者信息

Lindblom B, Holmlund G

机构信息

State Institute for Blood Group Serology, University Hospital, Linköping, Sweden.

出版信息

Gene Anal Tech. 1988 Sep-Oct;5(5):97-101. doi: 10.1016/0735-0651(88)90003-9.

DOI:10.1016/0735-0651(88)90003-9
PMID:2903845
Abstract

This paper describes a method for isolation of DNA from blood samples involving a rapid chemical disintegration of proteins with 8 M urea and with a minimum of exposure to phenol. The DNA is further desalted and purified on Sephadex G-25 prepacked disposable columns. DNA isolated in this way was pure enough to be immediately cleaved by restriction enzymes.

摘要

本文描述了一种从血液样本中分离DNA的方法，该方法涉及用8M尿素快速化学分解蛋白质，并尽量减少与苯酚的接触。DNA进一步在预装的一次性Sephadex G - 25柱上脱盐和纯化。以这种方式分离的DNA纯度足以立即被限制性内切酶切割。

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