Nahrevanian H, Gholizadeh J, Farahmand M, Assmar M, Sharifi K, Ayatollahi Mousavi S A, Abolhassani M
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
Scand J Clin Lab Invest. 2006;66(3):201-9. doi: 10.1080/00365510600565011.
Malaria has been prevalent for a long time in Iran and continues to be a health problem despite substantial control programs. In addition to numerous cytokines, nitric oxide (NO) is thought to be a key molecule and a novel target of malaria immunopathology.
The objective of this research was to measure reactive nitrogen intermediates (RNI) as stable metabolites of NO induction in plasma of malaria-infected patients in Iran. In this study, 235 blood samples from malaria patients and 80 blood samples from healthy controls were randomly collected from different malarial endemic provinces of Iran, located in southeastern (Sistan & Balouchestan, Hormozgan, Kerman) and northwestern (Ardabil) areas. The involvement of NO in malaria patients has been investigated by statistical analysis of RNI values. Griess micro assay (GMA) was used during Plasmodium vivax, P. falciparum and mixed infections, in order to evaluate whether RNI changes are related to the provincial areas, parasite strains, clinical symptoms and age and gender parameters.
The results showed a significant increase of RNI level in malaria patients compared with the control groups of Ardabil (p<0.01), Sistan & Balouchestan, Hormozgan and Kerman (p<0.001) provinces. The level of RNI was higher in mixed plasmodial infection than in single infection.
The high level of RNI was dependent on the type of infection, the plasmodia strain, the clinical symptoms, the age groups and the endemic provinces. Although, this study did not clarify the pathogenic and/or protective role of NO in malaria, our findings provide a novel immunoepidemiological aspect of basal NO production in patients with malaria in endemic areas in Iran.
疟疾在伊朗长期流行，尽管实施了大量防控项目，但仍是一个健康问题。除了众多细胞因子外，一氧化氮（NO）被认为是疟疾免疫病理学的关键分子和新靶点。
本研究的目的是测量伊朗疟疾感染患者血浆中作为NO诱导稳定代谢产物的活性氮中间体（RNI）。在本研究中，从伊朗东南部（锡斯坦-俾路支斯坦省、霍尔木兹甘省、克尔曼省）和西北部（阿尔达比勒省）不同疟疾流行省份随机采集了235份疟疾患者血样和80份健康对照血样。通过对RNI值进行统计分析，研究了NO在疟疾患者中的作用。在间日疟原虫、恶性疟原虫及混合感染期间，采用格里斯微量分析法（GMA），以评估RNI变化是否与省份地区、寄生虫菌株、临床症状以及年龄和性别参数有关。
结果显示，与阿尔达比勒省（p<0.01）、锡斯坦-俾路支斯坦省、霍尔木兹甘省和克尔曼省（p<0.001）的对照组相比，疟疾患者的RNI水平显著升高。混合疟原虫感染时的RNI水平高于单一感染。
RNI的高水平取决于感染类型、疟原虫菌株、临床症状、年龄组和流行省份。尽管本研究未阐明NO在疟疾中的致病和/或保护作用，但我们的研究结果为伊朗流行地区疟疾患者基础NO产生提供了一个新的免疫流行病学视角。
