伊朗疟疾流行地区的分子流行病学

Molecular epidemiology of malaria in endemic areas of Iran.

作者信息

Assmar Mehdi, Terhovanessian Arpy, Jahani Mohammad R, Nahrevanian Hossein, Amirkhani Aref, Piazak Norair, Esmaeili Ahmad Reza R, Farahmand Mahin, Zare Mohammad

机构信息

Department of Parasitology, Pasteur Institute of Iran, Tehran.

出版信息

Southeast Asian J Trop Med Public Health. 2003;34 Suppl 2:15-9.

PMID:19230565

Abstract

In this study, 333 blood samples of malaria cases positive by microscopic test (70.6% male and 29.4% female, p<0.05) were investigated. The group included 55 cases (16.52%) from Minab (Hormozgan Province), 116 cases (34.82%) from Iranshahr (Sistan-Baluchesta Province) and 162 cases (48.65%) from Kahnouj (Kerman Province). The results showed 244 cases (73.27%) were diagnosed as P. vivax, 87 cases (26.13%) P. falciparum and 2 cases (0.6%) showed a mixed infection of both Plasmodia. In a molecular study of the same samples using nested-polymerase chain reaction (nested-PCR), 185 cases (55.6%) were P. vivax, 50 cases (15%) P. falciparum and 95 cases (29%) both Plasmodia. Comparing the two methods used in this study, the highest rate of infection was found to be P. vivax. However, the rate of mixed infections (0.6% microscopy, 29% nested-PCR) varied and depended on the assay used. This indicated that the sensitivity of nested-PCR was greater than microscopic examination, especially for the detection of mixed-infections (p<0.05) in the current malaria epidemiology study.

摘要

在本研究中，对333份经显微镜检测呈阳性的疟疾病例血样进行了调查（男性占70.6%，女性占29.4%，p<0.05）。该组包括来自米纳卜（霍尔木兹甘省）的55例（16.52%）、来自伊朗沙赫尔（锡斯坦-俾路支斯坦省）的116例（34.82%）和来自卡努季（克尔曼省）的162例（48.65%）。结果显示，244例（73.27%）被诊断为间日疟原虫，87例（26.13%）为恶性疟原虫，2例（0.6%）显示两种疟原虫混合感染。在对相同样本进行的巢式聚合酶链反应（巢式PCR）分子研究中，185例（55.6%）为间日疟原虫，50例（15%）为恶性疟原虫，95例（29%）为两种疟原虫混合感染。比较本研究中使用的两种方法，发现感染率最高的是间日疟原虫。然而，混合感染率（显微镜检查为0.6%，巢式PCR为29%）有所不同，且取决于所使用的检测方法。这表明巢式PCR的敏感性高于显微镜检查，尤其是在当前疟疾流行病学研究中检测混合感染方面（p<0.05）。