Department of Animal Science, Universiti Putra Serdang, Malaysia.
Poult Sci. 2013 Apr;92(4):900-9. doi: 10.3382/ps.2012-02797.
Salmonella enterica serovar Enteritidis infection is a common concern in poultry production for its negative effects on growth as well as food safety for humans. Identification of molecular markers that are linked to resistance to Salmonella Enteritidis may lead to appropriate solutions to control Salmonella infection in chickens. This study investigated the association of candidate genes with resistance to Salmonella Enteritidis in young chickens. Two native breeds of Malaysian chickens, namely, Village Chickens and Red Junglefowl, were evaluated for bacterial colonization after Salmonella Enteritidis inoculation. Seven candidate genes were selected on the basis of their physiological role in immune response, as determined by prior studies in other genetic lines: natural resistance-associated protein 1 (NRAMP1), transforming growth factor β3 (TGFβ3), transforming growth factor β4 (TGFβ4), inhibitor of apoptosis protein 1 (IAP1), caspase 1 (CASP1), lipopolysaccharide-induced tumor necrosis factor (TNF) α factor (LITAF), and TNF-related apoptosis-inducing ligand (TRAIL). Polymerase chain reaction-RFLP was used to identify polymorphisms in the candidate genes; all genes exhibited polymorphisms in at least one breed. The NRAMP1-SacI polymorphism correlated with the differences in Salmonella Enteritidis load in the cecum (P = 0.002) and spleen (P = 0.01) of Village Chickens. Polymorphisms in the restriction sites of TGFβ3-BsrI, TGFβ4-MboII, and TRAIL-StyI were associated with Salmonella Enteritidis burden in the cecum, spleen, and liver of Village Chickens and Red Junglefowl (P < 0.05). These results indicate that the NRAMP1, TGFβ3, TGFβ4, and TRAIL genes are potential candidates for use in selection programs for increasing genetic resistance against Salmonella Enteritidis in native Malaysian chickens.
肠炎沙门氏菌感染是家禽生产中常见的问题，因为它会对生长产生负面影响，同时也对人类食品安全构成威胁。鉴定与抗肠炎沙门氏菌相关的分子标记可能会为控制鸡感染肠炎沙门氏菌提供适当的解决方案。本研究调查了候选基因与幼鸡抗肠炎沙门氏菌的关联性。本研究评估了两种马来西亚本土鸡品种，即 Village Chickens 和 Red Junglefowl，在接种肠炎沙门氏菌后的细菌定植情况。基于先前在其他遗传系中研究确定的免疫反应生理作用，选择了七个候选基因：天然抗性相关蛋白 1（NRAMP1）、转化生长因子 β3（TGFβ3）、转化生长因子 β4（TGFβ4）、凋亡抑制蛋白 1（IAP1）、半胱氨酸天冬氨酸蛋白酶 1（CASP1）、脂多糖诱导的肿瘤坏死因子（TNF）α因子（LITAF）和 TNF 相关凋亡诱导配体（TRAIL）。聚合酶链反应-RFLP 用于鉴定候选基因中的多态性；所有基因在至少一个品种中都表现出多态性。NRAMP1-SacI 多态性与肠炎沙门氏菌在 Village Chickens 的盲肠（P = 0.002）和脾脏（P = 0.01）中的负荷差异相关。TGFβ3-BsrI、TGFβ4-MboII 和 TRAIL-StyI 限制位点的多态性与肠炎沙门氏菌在 Village Chickens 和 Red Junglefowl 的盲肠、脾脏和肝脏中的负荷相关（P < 0.05）。这些结果表明，NRAMP1、TGFβ3、TGFβ4 和 TRAIL 基因是在马来西亚本土鸡中增加对肠炎沙门氏菌遗传抗性的选择计划的潜在候选基因。
