Rose John G, Lucarelli Mark J, Lemke Bradley N, Dortzbach Richard K, Boxrud Cynthia A, Obagi Suzan, Patel Sarit
Oculoplastics Service, Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, USA.
Ophthalmic Plast Reconstr Surg. 2006 May-Jun;22(3):195-200. doi: 10.1097/01.iop.0000217710.09941.10.
To perform a quantitative analysis of adipocyte viability after fat processing during autologous fat transfer, comparing the processing methods of washing, centrifuging, and sedimentation.
An experimental study was conducted in which 24 fat samples were obtained after processing from 22 patients undergoing autologous fat transfer. Histologic analysis of periodic acid-Schiff-stained specimens was then performed.
Cell counts per high-powered field of intact adipocytes and nucleated adipocytes and adipocyte cross-sectional area were significantly greater in samples processed by sedimentation, compared with those by centrifuging or washing.
Of the various processing techniques currently used during autologous fat transfer, sedimentation appears to yield a higher proportion of viable adipocytes than does washing or centrifuging.
在自体脂肪移植过程中，对脂肪处理后的脂肪细胞活力进行定量分析，比较洗涤、离心和沉淀这几种处理方法。
进行了一项实验研究，从22例接受自体脂肪移植的患者身上获取24份处理后的脂肪样本。然后对高碘酸-希夫染色标本进行组织学分析。
与离心或洗涤处理的样本相比，沉淀处理的样本中，每个高倍视野下完整脂肪细胞、有核脂肪细胞的细胞计数以及脂肪细胞横截面积均显著更高。
在目前自体脂肪移植过程中使用的各种处理技术中，沉淀法产生的存活脂肪细胞比例似乎高于洗涤法或离心法。
