Department of Neuroscience, Biomedicine and Movement, Human Anatomy and Histology Section, University of Verona, 37129 Verona, Italy.
Department of Computer Sciences, University of Verona, 37135 Verona, Italy.
Int J Mol Sci. 2020 Apr 27;21(9):3081. doi: 10.3390/ijms21093081.
Autologous fat grafting is a surgical technique in which adipose tissue is transferred from one area of the body to another, in order to reconstruct or regenerate damaged or injured tissues. Before reinjection, adipose tissue needs to be purified from blood and cellular debris to avoid inflammation and preserve the graft viability. To perform this purification, different enzymatic and mechanical methods can be used. In this study, we characterized in vitro the product of a closed automatic device based on mechanical disaggregation, named Rigenera, focusing on two sites of adipose tissue harvesting. At first, we optimized the Rigenera operating timing, demonstrating that 60 s of treatment allows a higher cellular yield, in terms of the cell number and growth rate. This result optimizes the mechanical disaggregation and it can increase the clinical efficiency of the final product. When comparing the extracted adipose samples from the thigh and abdomen, our results showed that the thigh provides a higher number of mesenchymal-like cells, with a faster replication rate and a higher ability to form colonies. We can conclude that by collecting adipose tissue from the thigh and treating it with the Rigenera device for 60 s, it is possible to obtain the most efficient product.
自体脂肪移植是一种将脂肪组织从身体的一个部位转移到另一个部位的手术技术，目的是重建或再生受损或受伤的组织。在重新注射之前，需要从血液和细胞碎片中纯化脂肪组织，以避免炎症并保持移植物的活力。为了进行这种纯化，可以使用不同的酶和机械方法。在这项研究中，我们对基于机械分散的封闭自动设备（名为 Rigenera）的体外产品进行了特征描述，重点关注两个脂肪组织采集部位。首先，我们优化了 Rigenera 的操作时间，结果表明 60 秒的处理可以提高细胞数量和生长速度方面的细胞产量。该结果优化了机械分散，并可以提高最终产品的临床效率。当比较来自大腿和腹部的提取脂肪样本时，我们的结果表明，大腿提供了更多的间充质样细胞，具有更快的复制率和更高的集落形成能力。我们可以得出结论，通过从大腿采集脂肪组织并使用 Rigenera 设备处理 60 秒，可以获得最有效的产品。
