Kallio Heini, Pastorekova Silvia, Pastorek Jaromir, Waheed Abdul, Sly William S, Mannisto Susanna, Heikinheimo Markku, Parkkila Seppo
Institute of Medical Technology, University of Tampere and Tampere University Hospital, Biokatu 8, FIN-33520 Tampere, Finland.
BMC Dev Biol. 2006 May 23;6:22. doi: 10.1186/1471-213X-6-22.
Of the thirteen active carbonic anhydrase (CA) isozymes, CA IX and XII have been linked to carcinogenesis. It has been suggested that these membrane-bound CAs participate in cancer cell invasion, which is facilitated by an acidic tumor cell environment. Since active cell migration is a characteristic feature of embryonic development, we set out to explore whether these isozymes are expressed in mouse embryos of different ages. The studies were focused on organogenesis stage.
Immunohistochemistry demonstrated that both CA IX and XII are present in several tissues of the developing mouse embryo during organogenesis. Staining for CA IX revealed a relatively wide distribution pattern with moderate signals in the brain, lung, pancreas and liver and weak signals in the kidney and stomach. The expression pattern of CA XII in the embryonic tissues was also relatively broad, although the intensity of immunostaining was weak in most tissues. The CA XII-positive tissues included the brain, where the most prominent staining was seen in the choroid plexus, and the stomach, pancreas, liver and kidney.
Membrane-bound CA isozymes IX and XII are expressed in various tissues during mouse organogenesis. These enzymes may regulate ion and pH homeostasis within the developing embryo.
在13种活性碳酸酐酶（CA）同工酶中，CA IX和CA XII与致癌作用有关。有人提出，这些膜结合型碳酸酐酶参与癌细胞侵袭，而酸性肿瘤细胞环境会促进这种侵袭。由于活跃的细胞迁移是胚胎发育的一个特征，我们着手探究这些同工酶在不同年龄小鼠胚胎中是否表达。研究集中在器官发生阶段。
免疫组织化学显示，在器官发生过程中，CA IX和CA XII均存在于发育中的小鼠胚胎的多个组织中。CA IX染色显示出相对广泛的分布模式，在脑、肺、胰腺和肝脏中有中等信号，在肾脏和胃中有弱信号。CA XII在胚胎组织中的表达模式也相对广泛，尽管在大多数组织中免疫染色强度较弱。CA XII阳性组织包括脑，脉络丛中染色最为明显，还有胃、胰腺、肝脏和肾脏。
膜结合型碳酸酐酶同工酶IX和XII在小鼠器官发生过程中在各种组织中表达。这些酶可能调节发育中胚胎内的离子和pH稳态。
