Borodinsky Laura N, Spitzer Nicholas C
Neurobiology Section, Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, CA 92093, USA.
Sci STKE. 2006 May 23;2006(336):pe22. doi: 10.1126/stke.3362006pe22.
Dynamic calcium signaling is a well-established precept in biology. Different cell types exhibit spontaneous as well as stimulus-triggered transient changes in the concentration of intracellular calcium. Does this behavior extend to other second messengers? Optical dissection of various signal transduction pathways with fluorescent reporter molecules that enable visualization of changes in concentration of other second messengers is well under way. Recent research using technologically refined probes provides improved temporal and spatial resolution of adenosine 3',5'-monophosphate (cAMP) dynamics to generate insights into the bidirectional interplay between intracellular fluctuations of cAMP and calcium. cAMP oscillations are generated in response to hormones, and cells can recognize and differentially respond to transient versus sustained changes in this second messenger. Second messenger reporters are now available to track multiple players and so provide a dynamic picture of signaling networks.
动态钙信号传导是生物学中一个已确立的概念。不同细胞类型在细胞内钙浓度方面表现出自发性以及刺激引发的瞬时变化。这种行为是否也适用于其他第二信使呢？利用荧光报告分子对各种信号转导途径进行光学剖析，从而能够可视化其他第二信使浓度的变化，这一工作正在顺利进行。最近使用技术上经过改进的探针进行的研究，提高了对3',5'-环磷酸腺苷（cAMP）动态变化的时间和空间分辨率，以便深入了解cAMP细胞内波动与钙之间的双向相互作用。cAMP振荡是对激素作出的反应而产生的，细胞能够识别并对这种第二信使的瞬时变化与持续变化作出不同反应。现在有了第二信使报告分子来追踪多个参与者，从而提供信号网络的动态图景。
