Sodré Fabio L, Castanho Vera S, Castilho Lucia N, de Barros-Mazon Silvia, de Faria Eliana C
Departamento de Patologia Clínica da Faculdade de Ciências Médicas Universidade Estadual de Campinas, São Paulo, Brasil.
J Clin Lab Anal. 2006;20(3):113-7. doi: 10.1002/jcla.20111.
This study evaluated the serum concentrations of lipids, lipoproteins, apolipoproteins, and high-density lipoprotein (HDL) subfractions in Brazilian adults. We analyzed the distribution of lipids in HDL2 and HDL3 in a normolipidemic population without evidence of established cardiovascular disease (CVD). A total of 93 males and 92 females, healthy and normolipidemic, volunteered to be submitted to a clinical examination, a blood collection, and to answer a questionnaire aimed at determining signs and symptoms of atherosclerotic disease. Their fasting plasma lipid, lipoproteins, apolipoproteins, and the cholesterol and triglyceride concentrations in HDL2 and HDL3, isolated by microultracentrifugation, were determined by enzymatic-colorimetric methods. The interpercentile intervals (2.5-97.5) for the population were established as being 5-18 mg/dL in men and 4-28 mg/dL in women for HDL2 cholesterol (HDL2chol) and 1-57 mg/dL in men and 2-61 mg/dL in women for HDL3 cholesterol (HDL3chol). HDL2 triglyceride levels (HDL2Tg) in men were 1-26 mg/dL and in women 2-28 mg/dL; moreover, the HDL3 triglyceride (HDL3Tg) intervals were established as 4-46 mg/dL for both sexes. The determination of reference ranges for lipids in HDL subfractions in populations without clinical atherosclerosis, is an useful tool for metabolic, diagnostic, and therapeutic approaches. We determined the intervals for HDL2chol, HDL3chol, HDL2Tg, and HDL3Tg. There were variations with sex and/or age for HDL2chol, HDL3chol, and HDL2Tg in the studied population.
本研究评估了巴西成年人血清中的脂质、脂蛋白、载脂蛋白以及高密度脂蛋白（HDL）亚组分。我们分析了无已确诊心血管疾病（CVD）证据的血脂正常人群中HDL2和HDL3的脂质分布情况。共有93名男性和92名女性，身体健康且血脂正常，自愿接受临床检查、采血，并回答一份旨在确定动脉粥样硬化疾病体征和症状的问卷。通过微超速离心法分离出HDL2和HDL3，采用酶比色法测定其空腹血浆脂质、脂蛋白、载脂蛋白以及胆固醇和甘油三酯浓度。该人群的百分位数区间（2.5 - 97.5）确定为：男性HDL2胆固醇（HDL2chol）为5 - 18mg/dL，女性为4 - 28mg/dL；男性HDL3胆固醇（HDL3chol）为1 - 57mg/dL，女性为2 - 61mg/dL。男性HDL2甘油三酯水平（HDL2Tg）为1 - 26mg/dL，女性为2 - 28mg/dL；此外，男女两性的HDL3甘油三酯（HDL3Tg）区间均确定为4 - 46mg/dL。对于无临床动脉粥样硬化的人群，确定HDL亚组分脂质的参考范围是代谢、诊断和治疗方法的有用工具。我们确定了HDL2chol、HDL3chol、HDL2Tg和HDL3Tg的区间。在所研究人群中，HDL2chol、HDL3chol和HDL2Tg存在性别和/或年龄差异。
