Liu Zhan, Jiao Qing-fang, You Chao, Che Yan-jun, Su Fang-zhong
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Chin J Traumatol. 2006 Jun;9(3):168-74.
To explore the effects of hyperbaric oxygen (HBO) treatment on the neuronal apoptosis at an earlier stage and the expressions of Cytochrome C (Cyt C), Bcl-2 (B-cell lymphoma-2 family) and Bax (Bcl-2 associated X protein) in rat brain tissues after traumatic brain injury (TBI).
Forty adult rats were divided into two groups, i.e., Group A (the rats with untreated TBI) and Group B (rats with HBO treatment after TBI). Sections of brain tissues of these two groups were then detected at 3, 6, 12, 24, 72 hours after TBI by immunohistochemistry and electronmicroscope, respectively.
HBO treatment could up-regulate the expression of Bcl-2 within 72 hours, reduce the release of Cyt C from mitochondria, attenuate the formation of dimeric Bax and alleviate the mitochondrial edema within 24 hours after TBI.
HBO treatment can alleviate neuronal apoptosis after TBI by reducing the release of Cyt C and the dimers of Bax and up-regulating the expression of Bcl-2.
探讨高压氧（HBO）治疗对创伤性脑损伤（TBI）大鼠脑组织早期神经元凋亡以及细胞色素C（Cyt C）、B细胞淋巴瘤-2（Bcl-2）和Bcl-2相关X蛋白（Bax）表达的影响。
将40只成年大鼠分为两组，即A组（未治疗的TBI大鼠）和B组（TBI后接受HBO治疗的大鼠）。然后分别在TBI后3、6、12、24、72小时通过免疫组织化学和电子显微镜检测这两组大鼠的脑组织切片。
HBO治疗可在72小时内上调Bcl-2的表达，减少TBI后24小时内细胞色素C从线粒体的释放，减轻二聚体Bax的形成并减轻线粒体水肿。
HBO治疗可通过减少细胞色素C和Bax二聚体的释放以及上调Bcl-2的表达来减轻TBI后的神经元凋亡。
