Mao-Ying Qi-Liang, Zhao Jun, Dong Zhi-Qiang, Wang Jun, Yu Jin, Yan Min-Fen, Zhang Yu-Qiu, Wu Gen-Cheng, Wang Yan-Qing
Department of Integrative Medicine and Neurobiology, Shanghai Medical College, Fudan University, China.
Biochem Biophys Res Commun. 2006 Jul 14;345(4):1292-8. doi: 10.1016/j.bbrc.2006.04.186. Epub 2006 May 15.
This study described a modified rat model of bone cancer pain. Syngeneic Walker 256 mammary gland carcinoma cells were injected into the tibia medullary cavity via intercondylar eminence. Series of tests were carried out including bone radiology, bone histology, ambulatory pain, thermal hyperalgesia, mechanical allodynia, weight bearing ability, and electrophysiological recording from primary afferent fibers. The rats inoculated with carcinoma cells showed significant ambulatory pain, mechanical allodynia, and reduction in weight bearing, as well as increased incidence of spontaneous activity in Abeta fibers in affected limb, whereas PBS (vehicle) or heat-killed cells (sham) injected rats showed no significant difference in comparison to normal rats. The pain hypersensitive behaviors were aggravated with time and destruction of bone. Interestingly, mechanical allodynia was also observed in the contralateral limb, indicating the involvement of 'mirror image' pain in bone cancer pain. In summary, the present study provided a useful and easily established rat model of bone cancer pain which will contribute to further study of the mechanisms underlying cancer pain.
本研究描述了一种改良的骨癌痛大鼠模型。将同基因的Walker 256乳腺癌细胞经髁间隆起注入胫骨骨髓腔。进行了一系列测试，包括骨放射学、骨组织学、动态疼痛、热痛觉过敏、机械性异常性疼痛、负重能力以及初级传入纤维的电生理记录。接种癌细胞的大鼠表现出明显的动态疼痛、机械性异常性疼痛和负重减轻，以及患侧肢体Aβ纤维自发活动发生率增加，而注射PBS（溶剂）或热灭活细胞（假手术）的大鼠与正常大鼠相比无显著差异。疼痛超敏行为随时间和骨破坏而加重。有趣的是，对侧肢体也观察到机械性异常性疼痛，表明“镜像”疼痛参与了骨癌痛。总之，本研究提供了一种有用且易于建立的骨癌痛大鼠模型，这将有助于进一步研究癌痛的潜在机制。
