Zhang Z, Artelt M, Burnet M, Trautmann K, Schluesener H J
Institute of Brain Research, University of Tuebingen, Calwer Str. 3, D-72076 Tuebingen, Germany.
Synovo GmbH, Paul-Ehrlich-Str. 15, D-72076 Tuebingen, Germany.
Neuroscience. 2006 Aug 25;141(2):637-644. doi: 10.1016/j.neuroscience.2006.04.027. Epub 2006 May 24.
Local inflammatory responses play an important role in mediating secondary tissue damage in traumatic brain injury. Characterization of leukocytic subpopulations contributing to the early infiltration of the damaged tissue might aid in further understanding of lesion development and contribute to definition of cellular targets for selective immunotherapy. In a rat traumatic brain injury model, significant CD8+ cell accumulation was observed 3 days post-injury. The CD8+ cells were strictly distributed to the pannecrotic areas and around the pannecrotic perimeter. The morphology, time course of accumulation and distribution of CD8+ cells were similar to that of reactive ED1+ and endothelial monocyte-activating polypeptide II+ microglia/macrophages, but different from W3/13+ T cells. Further double-labeling experiments confirmed that the major cellular sources of CD8 were reactive macrophages/microglia. Both the location of these CD8+ macrophages/microglia to the border of the pannecrosis and their co-expression of endothelial monocyte-activating polypeptide II and P2X4 receptor suggest they might have a central role in lesion development and might thus be candidates for development of immunotherapeutic, anti-inflammatory strategies.
局部炎症反应在介导创伤性脑损伤的继发性组织损伤中起重要作用。对导致受损组织早期浸润的白细胞亚群进行表征，可能有助于进一步了解损伤的发展，并有助于确定选择性免疫治疗的细胞靶点。在大鼠创伤性脑损伤模型中，损伤后3天观察到显著的CD8⁺细胞积聚。CD8⁺细胞严格分布于全坏死区域及其周边。CD8⁺细胞的形态、积聚的时间进程和分布与反应性ED1⁺和内皮单核细胞激活多肽II⁺小胶质细胞/巨噬细胞相似，但与W3/13⁺T细胞不同。进一步的双标记实验证实，CD8的主要细胞来源是反应性巨噬细胞/小胶质细胞。这些CD8⁺巨噬细胞/小胶质细胞位于全坏死边界的位置以及它们内皮单核细胞激活多肽II和P2X4受体的共表达表明，它们可能在损伤发展中起核心作用，因此可能是免疫治疗、抗炎策略开发的候选对象。
