Virakul P, Vahdat F, Joo H S, Zemjanis R
College of Veterinary Medicine, Department of Large Animal Clinical Science, University of Minnesota, St. Paul, MN 55108 USA.
Theriogenology. 1985 Apr;23(4):679-86. doi: 10.1016/0093-691x(85)90202-x.
Sera from 486 bovine fetuses, approximately 60 to 270 days of gestation, were collected at slaughter and tested for the presence of immunoglobulins (Ig). One hundred ten (27%) of the sera were positive for IgG and/or IgM. The earliest age at which fetuses tested positive for IgM and IgG was estimated to be 100 and 120 days, respectively. Ig concentration increased with increased age of the fetus. Sera that were positive for Ig were tested for the presence of specific antibodies to five different infectious agents. Bovine parvovirus antibodies were found in 99 of 110 sera (90%) by hemagglutination inhibition (HI) test. However, only 35 (31.8%) of these sera were positive by serum neutralization (SN) test. Antibodies to parainfluenza-3 virus were detected in 30 sera (27%) by HI test and in 20 sera (18%) by SN test. Five (4%) sera contained SN antibodies to bovine viral diarrhea virus. Only one (0.9%) serum sample contained SN antibodies to infectious bovine rhinotracheitis virus. None of the sera had antibodies against five Leptospira spp. Results of this study suggest that bovine parvovirus may be a potential cause of reproductive problems in cattle.
收集了486份来自妊娠约60至270天的牛胎儿的血清，在屠宰时采集并检测其中免疫球蛋白（Ig）的存在情况。110份（27%）血清的IgG和/或IgM呈阳性。胎儿IgM和IgG检测呈阳性的最早年龄分别估计为100天和120天。Ig浓度随胎儿年龄增长而增加。对Ig呈阳性的血清检测了针对五种不同传染原的特异性抗体。通过血凝抑制（HI）试验，在110份血清中的99份（90%）中发现了牛细小病毒抗体。然而，通过血清中和（SN）试验，这些血清中只有35份（31.8%）呈阳性。通过HI试验在30份血清（27%）中检测到副流感3病毒抗体，通过SN试验在20份血清（18%）中检测到该抗体。5份（4%）血清含有针对牛病毒性腹泻病毒的SN抗体。只有1份（0.9%）血清样本含有针对传染性牛鼻气管炎病毒的SN抗体。没有一份血清含有针对五种钩端螺旋体的抗体。本研究结果表明，牛细小病毒可能是牛繁殖问题的一个潜在原因。
