Sajiki Yamato, Konnai Satoru, Nishimori Asami, Okagawa Tomohiro, Maekawa Naoya, Goto Shinya, Nagano Masashi, Kohara Junko, Kitano Nana, Takahashi Toshihiko, Tajima Motoshi, Mekata Hirohisa, Horii Yoichiro, Murata Shiro, Ohashi Kazuhiko
Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
Animal Research Center, Agricultural Research Department, Hokkaido Research Organization, Shintoku, Hokkaido 081-0038, Japan.
J Vet Med Sci. 2017 Dec 22;79(12):2036-2039. doi: 10.1292/jvms.17-0391. Epub 2017 Nov 6.
Enzootic bovine leukemia is caused by the bovine leukemia virus (BLV). BLV is transmitted vertically or horizontally through the transfer of infected cells via direct contact, through milk, insect bites and contaminated iatrogenic procedures. However, we lacked direct evidence of intrauterine infection. The purpose of this study was to confirm intrauterine BLV infection in two pregnant dams with high viral load by cesarean delivery. BLV was detected in cord and placental blood, and the BLV in the newborns showed 100% nucleotide identity with the BLV-env sequence from the dams. Notably, a newborn was seropositive for BLV but had no colostral antibodies. In this study, we presented a direct evidence of intrauterine BLV transmission in pregnant dam with a high proviral load. These results could aid the development of BLV control measures targeting viral load.
地方流行性牛白血病由牛白血病病毒（BLV）引起。BLV通过受感染细胞的直接接触转移、乳汁、昆虫叮咬和受污染的医源性操作进行垂直或水平传播。然而，我们缺乏子宫内感染的直接证据。本研究的目的是通过剖宫产确认两只病毒载量高的怀孕母畜的子宫内BLV感染。在脐带血和胎盘血中检测到了BLV，并且新生儿体内的BLV与母畜的BLV-env序列显示出100%的核苷酸同一性。值得注意的是，一头新生牛对BLV血清学呈阳性，但没有初乳抗体。在本研究中，我们提供了子宫内BLV在病毒前体载量高的怀孕母畜中传播的直接证据。这些结果有助于制定针对病毒载量的BLV控制措施。
