Miller R B, Wilkie B N
Can J Comp Med. 1979 Jul;43(3):255-61.
In this investigation the indirect fluorescent antibody technique was used to titrate antibodies in bovine sera to parainfluenza 3, infectious bovine rhinotracheitis virus and bovine viral diarrhea virus. These results were compared to those determined on the same samples by hemagglutination inhibition for parainfluenza 3 virus and serum neutralization for bovine virus diarrhea and infectious bovine rhinotracheitis virus. The results of the serological methods agreed closely. The indirect fluorescent antibody technique is a rapid and sensitive method for detecting antibodies and the procedure lends itself to use in diagnostic laboratories. In addition to the above viruses the presence or absence of antibodies to bovine coronavirus and bovine adenovirus 3 were determined by the indirect fluorescent antibody technique in thoracic fluids from 100 aborted fetuses and 50 nonaborted fetuses. Results on these samples were not compared to hemagglutination inhibition or serum neutralization as the condition of fluid samples from aborted fetuses renders interpretation of such tests unreliable. Antibodies to one or more viruses were detected in 30 of the 100 aborted fetuses and in seven of the 50 nonaborted fetuses. Antibodies to more than one agent were detected in eleven of the 100 aborted and in one of the 50 nonaborted fetuses. Reasons for this occurrence and application of the test in determination of causes of abortion are discussed.
在本次研究中，采用间接荧光抗体技术对牛血清中抗副流感3型、传染性牛鼻气管炎病毒和牛病毒性腹泻病毒的抗体进行滴定。将这些结果与通过血凝抑制法检测副流感3型病毒抗体以及通过血清中和法检测牛病毒性腹泻病毒和传染性牛鼻气管炎病毒抗体的相同样本检测结果进行比较。血清学方法的结果高度一致。间接荧光抗体技术是一种快速且灵敏的抗体检测方法，该方法适用于诊断实验室。除上述病毒外，还通过间接荧光抗体技术检测了100例流产胎儿和50例未流产胎儿胸液中抗牛冠状病毒和牛腺病毒3型抗体的有无。由于流产胎儿的胸液样本状况使得此类检测结果的解读不可靠，因此未将这些样本的结果与血凝抑制或血清中和结果进行比较。在100例流产胎儿中有30例检测到针对一种或多种病毒的抗体，在50例未流产胎儿中有7例检测到抗体。在100例流产胎儿中有11例检测到针对不止一种病原体的抗体，在50例未流产胎儿中有1例检测到。文中讨论了这种情况发生的原因以及该检测在确定流产原因方面的应用。
