Verhage H G, Fazleabas A T, Jaffe R C, Murray M K
Department of Obstetrics and Gynecology, University of Illinois College of Medicine at Chicago, Chicago, IL 60680 USA; Department of Physiology and Biophysics (RCJ), University of Illinois College of Medicine at Chicago, Chicago, IL 60680 USA.
Theriogenology. 1988 Sep;30(3):469-76. doi: 10.1016/0093-691x(88)90196-3.
Media from cultured cat endometrial explants were analyzed for the presence of a previously characterized high molecular weight estrogen-dependent secretory protein (CUPED) by polyacrylamide gel electrophoresis and radioimmunoassay (RIA). Both L-[(3)H]-leucine and D-[(3)H]-glucosamine were incorporated into newly synthesized CUPED during the culture of endometrial explants obtained from estradiol-treated ovariectomized cats, but not during the culture of tissue obtained from untreated ovariectomized animals or estradiol-primed ovariectomized animals treated with progesterone. The addition of tunicamycin to the culture medium inhibited the synthesis and release of the glycosylated form of CUPED from endometrial explants obtained from estradiol-treated cats. These data demonstrate that CUPED is synthesized and released in vitro from endometrial explants obtained from estradiol-treated cats as a glycoprotein possessing N-linked oligosaccharide chains.
通过聚丙烯酰胺凝胶电泳和放射免疫测定法（RIA），分析培养的猫子宫内膜外植体的培养基中是否存在先前鉴定的高分子量雌激素依赖性分泌蛋白（CUPED）。在从经雌二醇处理的去卵巢猫获得的子宫内膜外植体培养过程中，L-[(3)H]-亮氨酸和D-[(3)H]-葡糖胺均掺入新合成的CUPED中，但在从未经处理的去卵巢动物或经孕酮处理的经雌二醇预处理的去卵巢动物获得的组织培养过程中则不然。向培养基中添加衣霉素可抑制从经雌二醇处理的猫获得的子宫内膜外植体中糖基化形式的CUPED的合成和释放。这些数据表明，CUPED作为一种具有N-连接寡糖链的糖蛋白，在体外从经雌二醇处理的猫获得的子宫内膜外植体中合成并释放。
