Breuel K F, Spitzer J C, Gimenez T, Henricks D M, Gray S L
Department of Animal Science Clemson University, Clemson, SC 29634-0361 USA.
Theriogenology. 1988 Sep;30(3):613-27. doi: 10.1016/0093-691x(88)90211-7.
Bovine jugular venous blood was collected, with and without heparin, and aliquoted into 140 12-ml tubes. Four subsamples (two heparinized and two coagulated) were centrifuged immediately (time zero) and plasma or serum was aspirated and stored at -20 degrees C. One-half of the remaining subsamples were stored at 4 degrees C and the other one-half at 25 degrees C (room temperature). At 1-h intervals (0 to 24 h), 6-h intervals (24 to 72 h) and at 96 and 120 h, four subsamples (heparinized and coagulated at both 4 degrees C and 25 degrees C) were centrifuged, plasma or serum was aspirated and stored at -20 degrees C. Whole blood incubation for 1 h at 25 degrees C reduced mean plasma and serum progesterone (P(4)) concentration (P<0.05). Similarly, whole blood incubation at 4 degrees C for 2 and 3 h, respectively, reduced mean plasma and serum P(4) concentration (P<0.05). No difference was found in mean P(4) concentration between plasma and serum samples harvested from whole blood incubated at 4 degrees C or 25 degrees C. Concentration of estradiol-17beta (E(2)) and estrone (E(1)) fluctuated over time, irrespective of holding temperature. There was a blood type, heparinized or coagulated, by time interaction (P<0.01) for both E(2) and E(1) concentrations It was concluded that incubation time and temperature between collection and centrifugation of bovine blood samples influenced the assayable P(4) concentration in both plasma and serum. In contrast, incubation temperature had no effect on assayable E(2) and E(1) concentrations, but assayable E(2) and E(1) over time were differentially affected, depending on whether plasma or serum was assayed.
采集了添加肝素和未添加肝素的牛颈静脉血，并将其分装到140个12毫升的试管中。四个子样本（两个添加肝素的和两个未添加肝素的）立即离心（时间为零），吸出血浆或血清并储存在-20℃。其余子样本的一半储存在4℃，另一半储存在25℃（室温）。在1小时间隔（0至24小时）、6小时间隔（24至72小时）以及96和120小时时，对四个子样本（4℃和25℃下添加肝素和未添加肝素的）进行离心，吸出血浆或血清并储存在-20℃。全血在25℃孵育1小时会降低血浆和血清孕酮（P(4)）的平均浓度（P<0.05）。同样，全血在4℃分别孵育2小时和3小时也会降低血浆和血清P(4)的平均浓度（P<0.05）。在4℃或25℃孵育的全血中采集的血浆和血清样本之间，P(4)的平均浓度没有差异。雌二醇-17β（E(2)）和雌酮（E(1)）的浓度随时间波动，与保存温度无关。对于E(2)和E(1)的浓度，存在血型（添加肝素或未添加肝素）与时间的交互作用（P<0.01）。得出的结论是，牛血样本采集和离心之间的孵育时间和温度会影响血浆和血清中可检测到的P(4)浓度。相比之下，孵育温度对可检测到的E(2)和E(1)浓度没有影响，但随着时间的推移，可检测到的E(2)和E(1)会受到不同影响，具体取决于检测的是血浆还是血清。
