Stapleton A, Moseley S, Stamm W E
Department of Medicine, University of Washington, Seattle.
J Infect Dis. 1991 Apr;163(4):773-9. doi: 10.1093/infdis/163.4.773.
To assess the prevalence of urovirulence determinants among Escherichia coli isolates from women with acute uncomplicated cystitis, 121 isolates from 87 women with first-episode or recurrent cystitis and 156 fecal isolates from 52 women without recent urinary tract infection were tested using DNA probes for P fimbriae, hemolysin, aerobactin, and diffuse adhesin and for expression of hemolysin and P and F adhesins. P fimbrial genotype (P = .002), hemolysin phenotype (P = .007), and the diffuse adhesin determinant (P = .03), but not aerobactin, were found more frequently in E. coli from women with acute cystitis, and expression of the F adhesin (41%) was more common than the P adhesin (24%; P = .001). E. coli isolates that caused cystitis in women using diaphragms had fewer virulence determinants than those from nonusers (P = .04), suggesting that diaphragm use may allow infection with less virulent E. coli.
为评估急性单纯性膀胱炎女性患者分离出的大肠杆菌中尿路毒力决定因素的流行情况，我们使用针对P菌毛、溶血素、气杆菌素和弥散黏附素的DNA探针，以及检测溶血素、P和F黏附素的表达，对87例首发或复发性膀胱炎女性患者的121株分离菌，以及52例近期无尿路感染女性患者的156株粪便分离菌进行了检测。发现P菌毛基因型（P = 0.002）、溶血素表型（P = 0.007）和弥散黏附素决定因素（P = 0.03）在急性膀胱炎女性患者的大肠杆菌中出现频率更高，而气杆菌素则不然，并且F黏附素的表达（41%）比P黏附素（24%；P = 0.001）更常见。使用子宫托的女性患者中引起膀胱炎的大肠杆菌分离株的毒力决定因素比未使用者少（P = 0.04），这表明使用子宫托可能会使毒性较低的大肠杆菌引发感染。
