Totey S M, Pawshe C H, Singh G P
Embryo Biotechnology Laboratory National Institute of Immunology Shahid Jeet Singh Marg New Delhi-110067, India.
Theriogenology. 1993 Apr;39(4):887-98. doi: 10.1016/0093-691x(93)90426-6.
A study was undertaken to assess the ability of spermatozoa from 6 buffalo bulls, at different levels of heparin and sperm concentrations, to achieve an acceptable level of fertilization in vitro. Frozen-thawed spermatozoa, 3 dosages of heparin (0, 10 and 100 ug/ml) in the presence and absence of penicillamine, hypotaurine and epinephrine (PHE), and 4 sperm concentrations (1 x 10(6), 2 x 10(6), 3 x 10(6) and 4 x 10(6) /ml) were studied using 3202 buffalo oocytes. The mean proportions of fertilized oocytes in the group treated with 10 ug/ml of heparin were significantly higher (P<0.05) with the semen of Bulls A, B and C (44.7 to 64.3%) than in medium devoid of heparin. An increase in the dosage of heparin from 10 ug/ml to 100 ug/ml reduced the overall fertilization rate. However, optimal fertilization (30.9%) at 100 ug/ml heparin was observed for semen from Bull D. Bulls E and F yielded the lowest fertilization rate (9.6 and 14.2%, respectively) at the above mentioned heparin dosage. Analysis of sperm density revealed that a concentration of 2 x 10(6) spermatozoa yielded optimal fertilization rates in vitro. Higher sperm concentrations (3 x 10(6) or 4 x 10(6)) resulted in higher oocyte penetration rates but gave rise to polyspermy.
开展了一项研究，以评估6头水牛公牛的精子在不同肝素水平和精子浓度下实现可接受体外受精水平的能力。使用3202枚水牛卵母细胞，研究了冻融精子、存在和不存在青霉胺、亚牛磺酸和肾上腺素（PHE）时的3种肝素剂量（0、10和100微克/毫升）以及4种精子浓度（1×10⁶、2×10⁶、3×10⁶和4×10⁶/毫升）。用10微克/毫升肝素处理的组中，公牛A、B和C的精液受精卵母细胞的平均比例（44.7%至64.3%）显著高于不含肝素的培养基（P<0.05）。肝素剂量从10微克/毫升增加到100微克/毫升会降低总体受精率。然而，观察到公牛D的精液在100微克/毫升肝素时的最佳受精率为30.9%。在上述肝素剂量下，公牛E和F的受精率最低（分别为9.6%和14.2%）。精子密度分析表明，2×10⁶精子浓度可产生最佳体外受精率。较高的精子浓度（3×10⁶或4×10⁶）导致较高的卵母细胞穿透率，但会引发多精受精。
