Vidament M, Dupere A M, Julienne P, Evain A, Noue P, Palmer E
Horse Institute, National Studs and I.N.R.A. 37380 Nouzilly, France.
Theriogenology. 1997 Oct 15;48(6):907-17. doi: 10.1016/s0093-691x(97)00319-1.
The freezability of stallion semen defined as the number of selected ejaculates/total number of ejaculates frozen from 161 different stallions was analyzed. Of the stallions, 19, 30, 27 and 24% had a freezability of 0%, 0 to 33%, 33 to 66%, over 66%, respectively In 85 different stallions, the correlation of freezability between first and second year was 0.60 (P < 0.001). The relationship between fertility with fresh and frozen semen and freezability was analyzed in 40 stallions whose freezability and fertility information was recorded during 5 years. There was a strong relationship between fertility of fresh semen and semen freezability (P < 0.001). However, the relationship between fertility of frozen semen and freezability was not as marked (P < 0.05). Analysis of the field fertility per cycle results when mares were bred with 300 or 150 x 10(6) total spermatozoa at different frequencies until ovulation indicated that mares that were inseminated 2 times or more per estrus show an improved fertility in comparison with mares inseminated only once (34%, n = 1576 vs 26%, n = 626; P < 0.001). Foaling rate when mares were inseminated with frozen semen (1858 mares during 8 breeding seasons) was mainly influenced by mare age (< 16 years: 54% vs >/= 16 years 42% p < 0.001). Date of first insemination (before May 15: 58% vs after May 15: 37%) also had a significant effect on foaling rate (P < 0.001).
对161匹不同种公马的精液冷冻能力进行了分析，精液冷冻能力定义为所选射精次数/冷冻射精总数。在这些种公马中，冷冻能力为0%、0至33%、33至66%、超过66%的分别占19%、30%、27%和24%。在85匹不同种公马中，第一年和第二年冷冻能力的相关性为0.60（P<0.001）。对40匹种公马在5年期间记录的冷冻能力和生育力信息进行分析，以研究新鲜精液和冷冻精液的生育力与冷冻能力之间的关系。新鲜精液的生育力与精液冷冻能力之间存在很强的关系（P<0.001）。然而，冷冻精液的生育力与冷冻能力之间的关系并不那么显著（P<0.05）。对母马在排卵前以不同频率用300或150×10⁶个总精子进行配种时每个周期的田间生育力分析表明，与仅配种一次的母马相比，每个发情期配种2次或更多次的母马生育力有所提高（34%，n = 1576对26%，n = 626；P<0.001）。用冷冻精液对母马进行配种时的产驹率（8个繁殖季节的1858匹母马）主要受母马年龄影响（<16岁：54%对≥16岁：42%，p<0.001）。首次配种日期（5月15日前：58%对5月15日后：37%）对产驹率也有显著影响（P<0.001）。
