Bayat Mohammad, Chelcheraghi Farzaneh, Piryaei Abbas, Rakhshan Mohammad, Mohseniefar Zhaleh, Rezaie Fatemesadat, Bayat Maryam, Shemshadi Hashem, Sadeghi Yousef
Cell and Molecular Biology Research Center, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
Med Sci Monit. 2006 Jun;12(6):BR201-7. Epub 2006 May 29.
The aim of this study was to clarify the histological, ultrastructural and biomechanical effects of pentoxifylline (PTX) on the survival of random skin flaps (RSFs) in rats.
MATERIAL/METHODS: Thirty male rats were randomly divided into experimental, sham and control groups. The experimental group received PTX 20 mg/kg/day, and the sham group received saline. A 20x70-mm RSF was made 30 days after the commencement of treatment for the three groups. PTX and saline were continued postoperatively for 7 days in the experimental and sham groups, respectively. On the seventh postoperative day, the surviving parts of the flaps were determined and examined through light and transmission electron microscopes. The wounds (incisions) on the margins of the flaps were evaluated histologically and biomechanically.
Analysis of variance showed that, in the experimental group, the mean of the surviving parts of the RSFs, fibroblast proliferation, collagen organization and granulation tissue of the wounds was significantly higher than in the sham and control groups (P=0.007, P=0.001, P=0.041, P=0.000, respectively). There were swollen mitochondria in the endothelium of the blood vessels of the surviving flap parts in the control and sham groups, whereas in the experimental group the mitochondria were normal.
Thirty days of pretreatment of RSFs with PTX significantly increased the survival of the flaps. PTX appeared to have healed wounds and reversed ultrastructural changes resulting from hypoxia in the blood vessel endothelium of the flaps.
本研究旨在阐明己酮可可碱（PTX）对大鼠随意皮瓣（RSF）存活的组织学、超微结构及生物力学影响。
材料/方法：30只雄性大鼠随机分为实验组、假手术组和对照组。实验组给予PTX 20mg/kg/天，假手术组给予生理盐水。三组在治疗开始30天后制作20×70mm的RSF。术后实验组和假手术组分别继续给予PTX和生理盐水7天。术后第7天，测定皮瓣存活部分并通过光学显微镜和透射电子显微镜检查。对皮瓣边缘的伤口（切口）进行组织学和生物力学评估。
方差分析显示，实验组中RSF存活部分的平均值、成纤维细胞增殖、胶原组织及伤口肉芽组织均显著高于假手术组和对照组（分别为P = 0.007、P = 0.001、P = 0.041、P = 0.000）。对照组和假手术组存活皮瓣部分血管内皮中的线粒体肿胀，而实验组中线粒体正常。
PTX对RSF进行30天预处理可显著提高皮瓣存活率。PTX似乎能够愈合伤口并逆转皮瓣血管内皮缺氧导致的超微结构变化。
