Pedretti Stephanie Luzia da Costa, Rena Cícero de Lima, Orellano Laura Alejandra Ariza, Lazari Marcela Guimarães de, Campos Paula Peixoto, Nunes Tarcizo Afonso
Fellow PhD degree, Postgraduate Program in Surgery and Ophthalmology, Department of Surgery, Medical School, Universidade Federal de Minas Gerais, Belo Horizonte - MG. Reproduction Biology Center, Universidade Federal de Juiz de Fora, Brazil. Substantive scientific and intellectual contributions to the study; conception and design; acquisition, analysis and interpretation of data; technical procedures; histopathological examinations; manuscript preparation and writing.
Reproduction Biology Center, Universidade Federal de Juiz de Fora, Brazil.
Acta Cir Bras. 2020 Dec 18;35(11):e301105. doi: 10.1590/ACB351105. eCollection 2020.
To assess the action of pentoxifylline, administered by subcutaneous route, on skin flap tissue repair in rats, and to verify the histological aspects and biomarkers.
Thirty-two male Wistar rats were divided into four groups: control (CT) and treated with pentoxifylline (P1, P3 and P5). Modified McFarlane technique flap was used. Ten days later, the animals were euthanized and the areas of viable and necrotic tissue were evaluated. Hematoxylin/eosin staining was used to assess the morphometric characteristics of the number of vessels and epithelial thickness. Picrosirius red was used to assess collagen density. VEGF and TGF-?1 levels on the skin flap and serum of the animals were measured by the ELISA method.
The macroscopic evaluation of the skin flap dimensions showed reduced necrotic tissue in the pentoxifylline (p < 0.05) treated groups. There was an increase in angiogenesis and reepithelization, demonstrated by analyses with an increased number of vessels (p < 0.05), VEGF and epithelial thickness. Fibrogenic effect showed decreased collagen density and TGF-β1 in the skin flap and serum.
The benefits of pentoxifylline administered by subcutaneous route, at dose 100 mg/kg, which was effective to improve the survival of skin flap by acting on tissue repair components, stimulating angiogenesis and reepithelization, in addition to reducing fibrogenesis.
评估经皮下途径给予己酮可可碱对大鼠皮瓣组织修复的作用，并验证其组织学特征和生物标志物。
将32只雄性Wistar大鼠分为四组：对照组（CT）和接受己酮可可碱治疗的组（P1、P3和P5）。采用改良的麦克法兰技术制作皮瓣。10天后，对动物实施安乐死并评估存活和坏死组织的面积。使用苏木精/伊红染色评估血管数量和上皮厚度的形态学特征。使用天狼星红染色评估胶原密度。通过酶联免疫吸附测定法测量动物皮瓣和血清中的血管内皮生长因子（VEGF）和转化生长因子-β1（TGF-β1）水平。
对皮瓣尺寸的宏观评估显示，己酮可可碱治疗组（p < 0.05）的坏死组织减少。血管生成和再上皮化增加，表现为血管数量增加（p < 0.05）、VEGF和上皮厚度增加。纤维化效应表现为皮瓣和血清中的胶原密度和TGF-β1降低。
皮下途径给予100 mg/kg剂量的己酮可可碱具有益处，它可通过作用于组织修复成分、刺激血管生成和再上皮化，以及减少纤维生成，有效提高皮瓣的存活率。
