Tomik B, Chwiej J, Szczerbowska-Boruchowska M, Lankosz M, Wójcik S, Adamek D, Falkenberg G, Bohic S, Simionovici A, Stegowski Z, Szczudlik A
Institute of Neurology, Jagiellonian University Medical College, Krakow, Poland.
Neurochem Res. 2006 Mar;31(3):321-31. doi: 10.1007/s11064-005-9030-6. Epub 2006 May 3.
The abnormalities of metallochemical reactions may contribute to the pathogenesis of Amyotrophic Lateral Sclerosis (ALS). In the present work, an investigation of the elemental composition of the gray matter, nerve cells and white matter from spinal cord tissues representing three ALS cases and five non-ALS controls was performed. This was done with the use of the synchrotron microbeam X-ray fluorescence technique (micro-SRXRF). The following elements were detected in the tissue sections: P, S, Cl, K, Ca, Fe, Cu, Zn and Br. A higher accumulation of Cl, K, Ca, Zn and Br was observed in the nerve cell bodies than in the surrounding tissue. Contrary to all other elements, Zn accumulation was lower in the white matter areas than in the gray matter ones. The results of quantitative analysis showed that there were no general abnormalities in the elemental accumulation between the ALS and the control group. However, for individual ALS cases such abnormalities were observed for the nerve cells. We also demonstrated differences in the elemental accumulation between the analyzed ALS cases.
金属化学反应异常可能与肌萎缩侧索硬化症（ALS）的发病机制有关。在本研究中，我们利用同步加速器微束X射线荧光技术（micro-SRXRF），对3例ALS病例和5例非ALS对照的脊髓组织中的灰质、神经细胞和白质的元素组成进行了研究。在组织切片中检测到以下元素：磷、硫、氯、钾、钙、铁、铜、锌和溴。观察到神经细胞体中氯、钾、钙、锌和溴的积累高于周围组织。与所有其他元素相反，白质区域的锌积累低于灰质区域。定量分析结果表明，ALS组和对照组之间在元素积累方面没有普遍异常。然而，在个别ALS病例的神经细胞中观察到了这种异常。我们还证明了所分析的ALS病例之间在元素积累上存在差异。
