Zucchi Eliana V M, Sartori Marair G F, Jármy-Di Bella Zsuzsanna K I, da Silva Ismael D C G, Rodrigues de Lima Geraldo, Girão Manoel J B C
Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil.
Menopause. 2006 May-Jun;13(3):500-5. doi: 10.1097/01.gme.0000183657.27937.c4.
To evaluate quantitatively, by means of immune histochemistry, the expression of the vascular endothelial growth factor (VEGF) in the bladder, vesicourethral junction, and urethra in normal, castrated adult rats and under estrogen administration.
Sixty adult virgin rats (Rattus norvergicus albinus, Rodentia, Mammalia) from the CEDEME-UNIFESP Animal House were used. Rats were divided into three groups. Group I comprised noncastrated rats, group II comprised oophorectomized rats, and group III comprised castrated rats administered 17beta-estradiol in the form of subcutaneous implants at the dose of 0.18 mg/implant for 30 days. After performing standard immunohistochemistry procedures, the intensity of the dark-brown color was used as the cytoplasmic protein expression of VEGF. Cells without this coloration or weakly stained were considered negative. percentile of VEGF expression was obtained by counting 1,000 cells per slide and establishing the ratio between positive and total cells.
The VEGF expression was uniform and similar along the urinary tract in group I. After castration, protein expression was almost absent in the bladder and was low in the vesicourethral junction and urethra. With estrogen replacement, very little of the expression was recovered in the bladder, and the reaction became evident in the vesicourethral junction and urethral sections.
The present study implies a potential relationship between VEGF and urinary tract physiology. The results suggest that there are quantitative differences in VEGF expression in these tissues depending on steroid hormone status.
通过免疫组织化学方法定量评估正常成年去势大鼠以及给予雌激素的成年去势大鼠膀胱、膀胱尿道连接处和尿道中血管内皮生长因子（VEGF）的表达。
使用来自CEDEME - UNIFESP动物房的60只成年未交配大鼠（挪威大白鼠，啮齿目，哺乳纲）。大鼠被分为三组。第一组为未去势大鼠，第二组为卵巢切除大鼠，第三组为去势大鼠，以0.18 mg/植入物的剂量皮下植入17β - 雌二醇，持续30天。在进行标准免疫组织化学程序后，深棕色的强度被用作VEGF的细胞质蛋白表达。没有这种染色或弱染色的细胞被视为阴性。通过每张载玻片计数1000个细胞并确定阳性细胞与总细胞的比例来获得VEGF表达的百分比。
第一组大鼠整个泌尿道的VEGF表达均匀且相似。去势后，膀胱中几乎没有蛋白表达，膀胱尿道连接处和尿道中的表达较低。给予雌激素替代后，膀胱中的表达仅少量恢复，而在膀胱尿道连接处和尿道切片中反应变得明显。
本研究表明VEGF与泌尿道生理学之间可能存在关联。结果表明，这些组织中VEGF的表达因类固醇激素状态而异，存在定量差异。
