Sampaio Maria D D, Jarmy-Di Bella Zsuzsanna I K, da Silva Ismael D C G, Santos Elaine T, de Souza Naiara C, Zucchi Eliana V M, Simões Manuel de J, Girão Manoel J B C, Sartori Marair G F
Department of Gynecology, Laboratory of Molecular Gynecology, Federal University of São Paulo (UNIFESP), Brazil.
Maturitas. 2009 Mar 20;62(3):317-20. doi: 10.1016/j.maturitas.2009.01.003. Epub 2009 Feb 8.
The purpose of this study was to investigate Vascular Endothelial Growth Factor Expression (VEGF) gene regulation by isoflavone in urinary tract tissues of castrated adult rats.
Forty-five adult rats, 90 days old, weighting 200 g were used, receiving a soy-free ration. The animals were castrated for drug administration for 30 days (125 microg genisteine/g body weight/day) and sacrificed, divided into three groups: Group I-control; Group II-started isoflavone administration on the 5th day after castration; Group III-started isoflavone administration on the 28th day after castration. RNA was isolated from each bladder and urethra. Determination of VEGF gene regulated by isoflavone was obtained using a semiquantitative RT-PCR and immunohistochemistry of total RNA isolated from bladder and urethra.
Our results demonstrate that isoflavone was able to upregulate mRNA level of the VEGF gene in the lower urinary tract of rats in Group II, where isoflavone administration was started at an early phase of estrogen deprivation, while in Group III, where isoflavone administration was started in the late phase of hypoestrogenism, did not show alteration of bladder and urethra VEGF gene expression, compared to placebo, maintaining the same level of the castrated rats without treatment.
The data indicate that VEGF expression in rats is also regulated by isoflavone in early phase of hypoestrogenism.
本研究旨在探讨异黄酮对去势成年大鼠尿路组织中血管内皮生长因子表达（VEGF）基因的调控作用。
选用45只90日龄、体重200 g的成年大鼠，给予不含大豆的日粮。对动物进行去势并给药30天（125微克染料木黄酮/克体重/天），然后处死，分为三组：第一组为对照组；第二组在去势后第5天开始给予异黄酮；第三组在去势后第28天开始给予异黄酮。从每个膀胱和尿道中分离RNA。使用半定量RT-PCR和从膀胱和尿道分离的总RNA的免疫组织化学方法来测定异黄酮调控的VEGF基因。
我们的结果表明，在雌激素缺乏早期开始给予异黄酮的第二组大鼠下尿路中，异黄酮能够上调VEGF基因的mRNA水平，而在低雌激素后期开始给予异黄酮的第三组中，与安慰剂相比，膀胱和尿道VEGF基因表达未显示改变，维持了未治疗去势大鼠的相同水平。
数据表明，低雌激素早期大鼠的VEGF表达也受异黄酮调控。
