Edwards M M, Robinson S R
School of Psychology, Psychiatry and Psychological Medicine, Monash University, Clayton, Victoria, Australia.
J Neural Transm (Vienna). 2006 Nov;113(11):1709-15. doi: 10.1007/s00702-006-0479-5. Epub 2006 Jun 1.
Neurodegenerative disorders such as Alzheimer's disease are characterized by increased intracellular and extracellular concentrations of the astrocytic proteins glial fibrillary acidic protein (GFAP) and S100B. The present study examined the potential contribution of tumor necrosis factor alpha (TNFalpha) to these changes by measuring astrocyte viability along with the intracellular and extracellular expression of GFAP and S100B following exposure to this cytokine. Although TNFalpha did not affect astrocyte viability, the extracellular levels of both proteins were increased three-fold with associated reductions in immunocytochemical labeling.
诸如阿尔茨海默病之类的神经退行性疾病的特征是星形胶质细胞蛋白胶质纤维酸性蛋白（GFAP）和S100B在细胞内和细胞外的浓度增加。本研究通过测量星形胶质细胞的活力以及在暴露于这种细胞因子后GFAP和S100B的细胞内和细胞外表达，来检测肿瘤坏死因子α（TNFα）对这些变化的潜在作用。尽管TNFα不影响星形胶质细胞的活力，但两种蛋白的细胞外水平均增加了三倍，同时免疫细胞化学标记有所减少。
