Ponath Gerald, Schettler Christiane, Kaestner Florian, Voigt Björn, Wentker Dennis, Arolt Volker, Rothermundt Matthias
Department of Psychiatry, Molecular Psychiatry Division, University of Muenster, Germany.
J Neuroimmunol. 2007 Mar;184(1-2):214-22. doi: 10.1016/j.jneuroim.2006.12.011. Epub 2007 Jan 24.
To find out if the astrocytic protein S100B involves its autocrine effects via RAGE we investigated the capacity of astrocytes to upregulate IL-6 and TNF-alpha expression by stimulation with S100B. The subcellular localization of RAGE expression at the cell surface membrane of cultured astrocytes was demonstrated by immunofluorescence microscopy, flow cytometry and Western blotting. S100B was able to stimulate IL-6 and TNF-alpha secretion in cultured astrocytes in a concentration- and time-dependent manner as shown by ELISA. S100B induced IL-6 and TNF-alpha secretion was blocked by the use of RAGE siRNA specific for knocking down RAGE expression.
为了探究星形胶质细胞蛋白S100B是否通过晚期糖基化终末产物受体（RAGE）发挥自分泌作用，我们研究了星形胶质细胞在S100B刺激下上调白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）表达的能力。通过免疫荧光显微镜、流式细胞术和蛋白质印迹法证实了培养的星形胶质细胞表面膜上RAGE表达的亚细胞定位。酶联免疫吸附测定（ELISA）结果显示，S100B能够以浓度和时间依赖性方式刺激培养的星形胶质细胞分泌IL-6和TNF-α。使用特异性敲低RAGE表达的RAGE小干扰RNA（siRNA）可阻断S100B诱导的IL-6和TNF-α分泌。
