Groppa M D, Ianuzzo M P, Tomaro M L, Benavides M P
Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Amino Acids. 2007 Feb;32(2):265-75. doi: 10.1007/s00726-006-0343-9.
The effect of different doses of cadmium and copper was studied in relation to growth and polyamine (Pas) metabolism in shoots of sunflower plants. Cadmium accumulated to higher levels than copper and shoot length was reduced by 0.5 and 1 mM Cd, but only by 1 mM Cu. At 1 mM of Cd or Cu, Put content increased 270% and 160% with Cd2+ and Cu2+, respectively. Spermidine (Spd) was modified only by 1 mM Cd, while spermine (Spm) declined after seeds germinated, increasing thereafter but only with 1 mM Cd or Cu (273% over the controls for Cd and 230% for Cu at day 16). Both ADC and ODC activities were increased by 1 mM Cd, whereas 1 mM Cu enhanced ADC activity, but reduced ODC activity at every concentration used. The role of Pas as markers of Cd or Cu toxicity is discussed.
研究了不同剂量的镉和铜对向日葵植株地上部生长及多胺（PA）代谢的影响。镉的积累水平高于铜，0.5 mM和1 mM的镉使地上部长度降低，但只有1 mM的铜使其降低。在1 mM的镉或铜处理下，腐胺（Put）含量分别随Cd2+和Cu2+增加了270%和160%。亚精胺（Spd）仅在1 mM镉处理下发生变化，而精胺（Spm）在种子萌发后下降，之后增加，但仅在1 mM镉或铜处理下增加（第16天时，镉处理组比对照组增加273%，铜处理组增加230%）。1 mM镉使精氨酸脱羧酶（ADC）和鸟氨酸脱羧酶（ODC）活性均增加，而1 mM铜增强了ADC活性，但在所用的每个浓度下均降低了ODC活性。文中讨论了多胺作为镉或铜毒性标志物的作用。
