Montag Markus, Schimming Thomas, van der Ven Hans
Department of Gynecological Endocrinology and Reproductive Medicine, University Clinics of Bonn, Germany.
Reprod Biomed Online. 2006 Apr;12(4):442-6. doi: 10.1016/s1472-6483(10)61996-7.
Recent studies using polarized light microscopy revealed a correlation between the presence of a spindle in human metaphase II meiotic oocytes and the fertilization rate following intracytoplasmic sperm injection (ICSI). Using a new spindle imaging system, it was possible to visualize the spindle image and the conventional light microscopic view of the oocyte simultaneously. Using this system, time-lapse studies of the meiotic cycle of human oocytes were performed. The video sequences showed that during the transition from metaphase I to metaphase II, the spindle completely disappears for approximately 40-60 min. These data support the idea that at least in some oocytes, the absence of the spindle is more likely an indicator for physiological progression through an important developmental stage of meiosis rather than a cellular disturbance. In view of the low fertilization rates of oocytes with absence of spindles as reported in the literature, the underlying problem could simply be the incorrect timing of ICSI.
最近使用偏振光显微镜的研究揭示了人类减数分裂II中期卵母细胞中纺锤体的存在与胞浆内单精子注射（ICSI）后的受精率之间的相关性。使用一种新的纺锤体成像系统，可以同时可视化纺锤体图像和卵母细胞的传统光学显微镜视图。利用该系统，对人类卵母细胞的减数分裂周期进行了延时研究。视频序列显示，在从减数分裂I中期向减数分裂II中期转变的过程中，纺锤体完全消失约40 - 60分钟。这些数据支持了这样一种观点，即至少在某些卵母细胞中，纺锤体的缺失更可能是减数分裂重要发育阶段生理进程的一个指标，而非细胞紊乱。鉴于文献中报道的无纺锤体卵母细胞受精率较低，潜在问题可能仅仅是ICSI的时机不正确。
