García-Oro Sabela, Rey María Isabel, Rodríguez Marta, Durán Ángel, Devesa Roque, Valverde Diana
Equipo Ron - Hospital Quirón A Coruña, C/Londres 2, 15009, A Coruña, Spain.
Department Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, Campus, As Lagoas Marcosende S/N, 36310, Vigo, Spain.
J Assist Reprod Genet. 2017 May;34(5):617-625. doi: 10.1007/s10815-017-0897-3. Epub 2017 Mar 9.
We evaluated the relationship between meiotic spindle characteristics and in vitro fertilization cycle outcome.
Five hundred sixty-nine oocytes from 86 in vitro fertilization cycles were analyzed for fertilization and subsequent implantation rates. Oocytes were assessed for maturation status. The oocytes and embryos were cultured in sequential and nonsequential media (G Series, Vitrolife, Sweden) and incubated in 6% CO, 5% O at 37 °C. Two hours following oocyte decumulation (38-39 h post-hCG/GnRH administration) and prior to microinjection, the structure of the meiotic spindle was assessed using the Oosight Imaging System (CRI, UK).
Four hundred fifty-six oocytes (80.5%) had a visible meiotic spindle, 82 (14.7%) had no meiotic spindle, and 31 (5.5%) were in telophase I. Oocytes exhibiting a meiotic spindle had a significantly higher fertilization rate and a lower rate of abnormal fertilization. Implantation data were obtained for 195 of the embryos transferred. The implantation rate for embryos derived from oocytes with a meiotic spindle was 32.9%, while in embryos originating from oocytes without a meiotic spindle and oocytes in telophase, this value dropped significantly (8.8 and 0%, respectively). To determine the correlation between retardance values and implantation rate for each oocyte, we established four groups, finding a range of retardance values with significantly higher implantation rates (27.5, 21, 29.3, and 53.8%, respectively).
Meiotic spindle imaging may be a valuable tool for prediction of oocyte quality, and retardance values of meiotic spindles, together with classical morphological classification, can be useful to select embryos with a higher implantation potential.
我们评估了减数分裂纺锤体特征与体外受精周期结局之间的关系。
对来自86个体外受精周期的569个卵母细胞进行受精和后续着床率分析。评估卵母细胞的成熟状态。卵母细胞和胚胎在序贯和非序贯培养基（G系列，Vitrolife，瑞典）中培养，并在37℃、6%二氧化碳、5%氧气条件下孵育。在卵母细胞去卵丘后2小时（注射hCG/GnRH后38 - 39小时）且在显微注射前，使用Oosight成像系统（CRI，英国）评估减数分裂纺锤体的结构。
456个卵母细胞（80.5%）有可见的减数分裂纺锤体，82个（14.7%）没有减数分裂纺锤体，31个（5.5%）处于减数第一次分裂末期。显示有减数分裂纺锤体的卵母细胞受精率显著更高，异常受精率更低。对195个移植胚胎获取了着床数据。来自有减数分裂纺锤体的卵母细胞的胚胎着床率为32.9%，而来自没有减数分裂纺锤体的卵母细胞和处于减数第一次分裂末期的卵母细胞的胚胎，该值显著下降（分别为8.8%和0%）。为确定每个卵母细胞的延迟值与着床率之间的相关性，我们建立了四组，发现延迟值范围对应的着床率显著更高（分别为27.5%、21%、29.3%和53.8%）。
减数分裂纺锤体成像可能是预测卵母细胞质量的有价值工具，减数分裂纺锤体的延迟值与经典形态学分类一起，可用于选择具有更高着床潜力的胚胎。
