Sylwia Golawska, Leszczynski Bogumil, Wieslaw Oleszek
Department of Biochemistry, University of Podlasie, ul. Prusa 12, 08-110 Siedlce, Poland.
J Insect Physiol. 2006 Jul;52(7):737-43. doi: 10.1016/j.jinsphys.2006.04.001. Epub 2006 Apr 25.
Pea aphid fed on a high-saponin line of alfalfa showed reduction of reproduction and survival, and disturbance in development of its population. This line negatively influenced aphid probing behaviour, particularly prolonging the non-probing period and probing of the peripheral tissues (epidermis and mesophyll) and shortening the period of phloem sap ingestion. The high-saponin line of alfalfa differed from the low-saponin one by the presence of zanhic acid tridesmoside and a higher level of 3-GlcA,28-AraRhaXyl medicagenic acid glycoside. The saponins incorporated into sucrose-agarose gels significantly reduced number of the aphid probes into the gels and extended their duration in comparison to the control gels (without tested compounds). Role of zanhic acid tridesmoside and 3-GlcA,28-AraRhaXyl medicagenic acid glycoside as potential factors for partial resistance of alfalfa towards the pea aphid is discussed.
以高皂素含量的苜蓿品系为食的豌豆蚜，其繁殖和生存能力下降，种群发育受到干扰。该品系对蚜虫的取食行为产生负面影响，特别是延长了非取食期和对周边组织（表皮和叶肉）的取食时间，并缩短了韧皮部汁液摄取期。高皂素含量的苜蓿品系与低皂素含量的品系不同，前者含有三糖基酯酸和较高水平的3 - 葡萄糖醛酸、28 - 阿拉伯糖鼠李糖木糖苜蓿酸糖苷。与对照凝胶（不含测试化合物）相比，掺入蔗糖 - 琼脂糖凝胶中的皂素显著减少了蚜虫对凝胶的刺探次数，并延长了刺探持续时间。文中讨论了三糖基酯酸和三糖基酯酸糖苷作为苜蓿对豌豆蚜部分抗性潜在因素的作用。
