Saito S, Okui K, Tokino T, Oshimura M, Nakamura Y
Division of Biochemistry, Cancer Institute, Tokyo, Japan.
Am J Hum Genet. 1992 Jan;50(1):65-70.
We have isolated 68 new RFLP markers on human chromosome 6. Of these, 64 were localized on chromosomal bands by the fluorescent in-situ hybridization (FISH) method, 25 on the short arm and 39 on the long arm. Their distribution was uneven; the markers were localized predominantly in regions of R-positive banding. Eleven markers defined VNTR loci. This expanded collection of DNA markers will contribute to high-resolution linkage mapping of genes causing inherited disorders and will provide useful reagents for isolation of putative tumor-suppressor genes on chromosome 6 that appear to be involved in malignancies. Furthermore, the new markers will be guideposts for detailed linkage and physical maps of this chromosome.
我们在人类6号染色体上分离出了68个新的限制性片段长度多态性（RFLP）标记。其中，64个通过荧光原位杂交（FISH）方法定位在染色体带上，25个在短臂上，39个在长臂上。它们的分布不均匀；这些标记主要定位在R阳性带区域。11个标记定义了可变数目串联重复序列（VNTR）位点。这个扩展的DNA标记集合将有助于对导致遗传性疾病的基因进行高分辨率连锁图谱分析，并将为分离6号染色体上似乎与恶性肿瘤有关的假定肿瘤抑制基因提供有用的试剂。此外，这些新标记将成为该染色体详细连锁图谱和物理图谱的路标。
