人类精液中的乳酸脱氢酶同工酶。
Lactate dehydrogenase isoenzymes of human semen.
作者信息
Clausen J, Ovlisen B
机构信息
University Department of Biochemistry and University Department of Obstetrics and Gynaecology, Rigshospitalet, Copenhagen, Denmark.
出版信息
Biochem J. 1965 Nov;97(2):513-7. doi: 10.1042/bj0970513.
Abstract
- A lactate dehydrogenase isoenzyme present in human spermatozoa and semen was isolated and characterized biochemically in term of its pH for optimum activity and by means of K(m) values for lactate, NAD(+) and NAD analogues. The results were compared with those obtained with the human heart-type and the liver-type lactate dehydrogenase isoenzymes. 2. The enzyme was characterized by its resistance to digestion with different proteolytic enzymes. The time for 50% digestion in terms of residual dehydrogenase activity was compared with times obtained for the H(4)- and M(4)-types.
摘要
- 从人类精子和精液中分离出一种乳酸脱氢酶同工酶，并根据其最适活性的pH值以及乳酸、NAD⁺和NAD类似物的米氏常数（Km值）进行了生化特性鉴定。将结果与人类心脏型和肝脏型乳酸脱氢酶同工酶的结果进行了比较。2. 该酶的特点是对不同的蛋白水解酶具有抗性。根据残留脱氢酶活性比较了50%消化所需的时间与H₄型和M₄型的时间。
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本文引用的文献
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Nature and Development of Lactic Dehydrogenases: The two major types of this enzyme form molecular hybrids which change in makeup during development.乳酸脱氢酶的性质与发育：该酶的两种主要类型形成分子杂交体，其组成在发育过程中会发生变化。
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Lactic and Malic Dehydrogenases in Human Spermatozoa.人精子中的乳酸脱氢酶和苹果酸脱氢酶。
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Lactate Dehydrogenases in Human Testes.人睾丸中的乳酸脱氢酶。
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