Burgess Barry L, Rao Nagesh P, Eskin Ascia, Nelson Stanley F, McCannel Tara A
Department of Ophthalmology, University of California, Los Angeles, The Jules Stein Eye Institute, Los Angeles, CA, USA.
Mol Vis. 2011 Feb 25;17:607-15.
To report three low-passage cell lines from primary choroidal melanoma with metastatic outcome, which were stable for cytogenetic patterns and expression profiles of the primary melanoma.
In patients with choroidal melanoma, transscleral fine needle aspiration biopsy (FNAB) was performed immediately before plaque placement for (125)iodine brachytherapy or immediately after enucleation. Cells were examined for cytopathology, evaluated by fluorescence in-situ hybridization (FISH) for the centromere of chromosome 3, analyzed by 250K whole genome Mapping Array and U133 plus 2.0 Expression Array, and placed in cell culture. At passage 3, the cell lines were analyzed by Mapping Array and Expression Array.
Three cell lines were propagated from primary choroidal melanomas in three patients who subsequently developed metastasis. Two cell lines were stable for the entire chromosomal aberration pattern of the respective primary tumor. In the third, necrotic material from the biopsy prevented further analysis, yet resulted in a stable cell line. Each cell line had chromosome 3 loss, 6q loss, 8p loss, multiple 8q gain, and 16q loss. Additionally, two cell lines had chromosome 6p gain. Two cell lines had RNA expression profiles similar to the respective primary tumors; the third cell line had a similar RNA expression profile relative to the other two cell lines.
FNAB of primary choroidal melanomas resulted in highly characterized, low-passage cell lines, which were stable for the cytogenetic patterns and expression profiles found in the primary tumor. These cell lines represent novel tools for the study of metastatic choroidal melanoma biology.
报告三例来自原发性脉络膜黑色素瘤且具有转移结果的低传代细胞系，这些细胞系在原发性黑色素瘤的细胞遗传学模式和表达谱方面具有稳定性。
对于脉络膜黑色素瘤患者，在进行（125）碘近距离放疗的斑块放置前或眼球摘除术后立即进行经巩膜细针穿刺活检（FNAB）。对细胞进行细胞病理学检查，通过荧光原位杂交（FISH）评估3号染色体着丝粒，使用250K全基因组图谱阵列和U133 plus 2.0表达阵列进行分析，并置于细胞培养中。在第3代时，通过图谱阵列和表达阵列对细胞系进行分析。
从三名随后发生转移的患者的原发性脉络膜黑色素瘤中培养出三株细胞系。两株细胞系对于各自原发性肿瘤的整个染色体畸变模式具有稳定性。在第三例中，活检的坏死物质妨碍了进一步分析，但仍产生了一个稳定的细胞系。每个细胞系都有3号染色体缺失、6q缺失、8p缺失、多个8q增益和16q缺失。此外，两株细胞系有6p增益。两株细胞系的RNA表达谱与各自的原发性肿瘤相似；第三株细胞系相对于其他两株细胞系具有相似的RNA表达谱。
原发性脉络膜黑色素瘤的FNAB产生了高度特征化的低传代细胞系，这些细胞系在原发性肿瘤中发现的细胞遗传学模式和表达谱方面具有稳定性。这些细胞系代表了研究转移性脉络膜黑色素瘤生物学的新工具。
