Ratcliff R M, Lanser J A, Manning P A, Heuzenroeder M W
Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia.
J Clin Microbiol. 1998 Jun;36(6):1560-7. doi: 10.1128/JCM.36.6.1560-1567.1998.
The identification and speciation of strains of Legionella is often difficult, and even the more successful chromatographic classification techniques have struggled to discriminate newly described species. A sequence-based genotypic classification scheme is reported, targeting approximately 700 nucleotide bases of the mip gene and utilizing gene amplification and direct amplicon sequencing. With the exception of Legionella geestiana, for which an amplicon was not produced, the scheme clearly and unambiguously discriminated among the remaining 39 Legionella species and correctly grouped 26 additional serogroup and reference strains within those species. Additionally, the genotypic classification of approximately 150 wild strains from several continents was consistent with their phenotypic classification, with the exception of a few strains where serological cross-reactivity was complex, potentially confusing the latter classification. Strains thought to represent currently uncharacterized species were also found to be genotypically unique. The scheme is technically simple for a laboratory with even basic molecular capabilities and equipment, if access to a sequencing laboratory is available.
嗜肺军团菌菌株的鉴定和分类往往很困难，即使是较为成功的色谱分类技术也难以区分新描述的物种。本文报道了一种基于序列的基因型分类方案，该方案针对mip基因约700个核苷酸碱基，利用基因扩增和直接扩增子测序技术。除未产生扩增子的吉氏军团菌外，该方案清晰明确地区分了其余39种嗜肺军团菌，并将另外26个血清群和参考菌株正确地归为这些物种。此外，来自几大洲的约150株野生菌株的基因型分类与表型分类一致，但有少数菌株的血清学交叉反应复杂，可能会混淆后者的分类。被认为代表目前未鉴定物种的菌株在基因型上也具有独特性。对于一个即使只有基本分子能力和设备的实验室来说，如果能够使用测序实验室，该方案在技术上是简单的。
