Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Department of Pharmacology and Toxicology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Behav Neurol. 2022 Apr 26;2022:1877761. doi: 10.1155/2022/1877761. eCollection 2022.
Pregnant Wistar rats were randomly assigned into five groups: control, NP (25 mg/kg), NP (25 mg/kg)+MLT (10 mg/kg), NP (25 mg/kg)+MLT (20 mg/kg), and MLT (20 mg/kg). The duration of treatment was 21 days from gestation time. Morris water maze was used to assess learning and memory. NP concentrations of serum and testicular tissue were measured by HPLC. Histological analysis of testicular tissues was done by H&E staining.
Behavioral study showed that NP does not impair learning and memory in first-generation rats. Histomorphometric results showed that NP can significantly reduce the cross-sectional area of the seminiferous tubules and the epithelium, the diameter and number of seminiferous tubules, the thickness of the epithelium, and the number of spermatocytes and spermatogonia compared to other groups. MLT reversed the NP-induced histomorphometric. Also, it changes and increased the activity of superoxide dismutase (SOD), total antioxidant capacity (TAC), and catalase (CAT). The level of malondialdehyde (MDA) significantly decreased in MLT-treated groups compared with the NP group.
Our finding showed that MLT enhanced the learning process and reduced NP-induced testicular tissue damage through its antioxidants and cytoprotective effects.
将怀孕的 Wistar 大鼠随机分为五组：对照组、NP（25mg/kg）组、NP（25mg/kg）+MLT（10mg/kg）组、NP（25mg/kg）+MLT（20mg/kg）组和 MLT（20mg/kg）组。治疗时间从妊娠开始持续 21 天。采用 Morris 水迷宫评估学习和记忆能力。通过 HPLC 测量血清和睾丸组织中的 NP 浓度。用 H&E 染色对睾丸组织进行组织学分析。
行为研究表明，NP 不会损害第一代大鼠的学习和记忆能力。组织形态计量学结果显示，与其他组相比，NP 可显著降低曲细精管和上皮的横截面积、曲细精管直径和数量、上皮厚度以及精母细胞和精原细胞数量。MLT 逆转了 NP 诱导的组织形态计量学变化，并增加了超氧化物歧化酶（SOD）、总抗氧化能力（TAC）和过氧化氢酶（CAT）的活性。与 NP 组相比，MLT 治疗组丙二醛（MDA）水平显著降低。
我们的研究结果表明，MLT 通过其抗氧化和细胞保护作用增强了学习过程并减轻了 NP 诱导的睾丸组织损伤。
