Atambay Metin, Karabulut Aysun Bay, Aycan Ozlem Makbule, Kilic Eser, Yazar Suleyman, Saraymen Recep, Karaman Ulku, Daldal Nilgun
Department of Parasitology, Inonu University, Medical Faculty, Malatya, Turkey.
Natl Med J India. 2006 Mar-Apr;19(2):75-7.
Dust-mites are present in our homes, feed on dead exfoliated skin and other organic material. It is also known that oxidative stress may lead to cellular damage that can be confirmed by markers of cellular disruption. Oxidative stress in various infective processes has been documented. We investigated whether house dust-mites cause oxidative stress in patients.
Products of lipid peroxidation in erythrocytes and lymphocytes were assessed by measuring malondialdehyde concentration.
Our results showed that patients who had a positive skin test for dust-mite antigens and had dust-mites present in their houses (dust-mite positive) had increased erythrocyte malondialdehyde levels (62.39 [18.56] nmol/g-Hb) compared with those who were skin test positive, dust-mite negative (45.45 [10.82]) or skin test negative, dust-mite negative (42.20 [5.68]). They also had significantly higher levels of lymphocyte malondialdehyde (4.22 [0.55] nmol/g-protein) compared with those who were skin test positive, dust-mite negative (3.46 [0.29]) or skin test negative, dust-mite negative (1.25 [0.31]; p <0.05). However, there was no statistically significant difference between the malondialdehyde levels of dust-mite negative/skin test positive and dust-mite negative/skin test negative patients.
Increased malondialdehyde activity in lymphocytes and erythrocytes in the dust-mite positive/skin test positive group shows the presence of the oxidative stress in patients with dust-mite infestation.
尘螨存在于我们家中，以死皮屑和其他有机物质为食。已知氧化应激可能导致细胞损伤，这可通过细胞破坏的标志物来证实。各种感染过程中的氧化应激已有文献记载。我们调查了屋尘螨是否会导致患者出现氧化应激。
通过测量丙二醛浓度来评估红细胞和淋巴细胞中脂质过氧化的产物。
我们的结果显示，对尘螨抗原皮肤试验呈阳性且家中有尘螨（尘螨阳性）的患者，其红细胞丙二醛水平（62.39[18.56]nmol/g-Hb）高于皮肤试验阳性、尘螨阴性的患者（45.45[10.82]）或皮肤试验阴性、尘螨阴性的患者（42.20[5.68]）。与皮肤试验阳性、尘螨阴性的患者（3.46[0.29]）或皮肤试验阴性、尘螨阴性的患者（1.25[0.31]；p<0.05）相比，他们的淋巴细胞丙二醛水平也显著更高（4.22[0.55]nmol/g-蛋白质）。然而，尘螨阴性/皮肤试验阳性患者和尘螨阴性/皮肤试验阴性患者的丙二醛水平之间没有统计学上的显著差异。
尘螨阳性/皮肤试验阳性组中淋巴细胞和红细胞丙二醛活性增加表明尘螨感染患者存在氧化应激。
