Chang Yih-Hsin, Wang Lina, Lee Ming-Shih, Cheng Chun-Wen, Wu Chun-Ying, Shiau Ming-Yuh
School of Medical Laboratory and Biotechnology, Shan Medical University, Taichung, Taiwan, Republic of China.
Mt Sinai J Med. 2006 May;73(3):622-6.
Helicobacter pylor i infection is closely associated with gastroduodenal diseases. H. pylori infection with different vacA and cagA genotypes may result in divergent consequences. The aim of the present study was to investigate the prevalence of H. pylori infection and the correlation between cagA and vacA genotypes with the consequences of H. pylori infection in Taiwan.
Genomic DNA from 97 gastric biopsies of patients with various gastroduodenal diseases was collected, and the prevalence of H. pylori infection, cagA genotypes and vacA genotypes, was analyzed by polymerase chain reaction. In addition, the correlations between cagA and vacA genotypes and the consequences of H. pylori-infection were statistically examined.
Our results indicated that 57.7% of this sample of patients with gastroduodenal diseases were infected with H. pylori. Prevalence of cagA(+) strain in H. pylori -infected patients was 71.4%. All of the genotypes of the cagA(+) H. pylori strains among our patients were type A. Prevalence of vacA signal region s1 and middle region m2 genotype in H. pylori- infected patients was 98.2% and 53.6%, respectively.
Our study demonstrated that individuals infected with H. pylori strains that carried cagA gene and vacA s1/m2 genotypes were associated with the development of gastroduodenal diseases, compared to those infected with cagA(-) gene and vacA(-) H. pylori strains.
幽门螺杆菌感染与胃十二指肠疾病密切相关。不同vacA和cagA基因型的幽门螺杆菌感染可能导致不同的后果。本研究的目的是调查台湾地区幽门螺杆菌感染的患病率，以及cagA和vacA基因型与幽门螺杆菌感染后果之间的相关性。
收集97例患有各种胃十二指肠疾病患者的胃活检组织中的基因组DNA，通过聚合酶链反应分析幽门螺杆菌感染的患病率、cagA基因型和vacA基因型。此外，对cagA和vacA基因型与幽门螺杆菌感染后果之间的相关性进行统计学检验。
我们的结果表明，该胃十二指肠疾病患者样本中57.7%感染了幽门螺杆菌。幽门螺杆菌感染患者中cagA(+)菌株的患病率为71.4%。我们患者中所有cagA(+)幽门螺杆菌菌株的基因型均为A型。幽门螺杆菌感染患者中vacA信号区s1和中区m2基因型的患病率分别为98.2%和53.6%。
我们的研究表明，与感染cagA(-)基因和vacA(-)幽门螺杆菌菌株的个体相比，感染携带cagA基因和vacA s1/m2基因型幽门螺杆菌菌株的个体与胃十二指肠疾病的发生有关。
