泰国消化不良患者中幽门螺杆菌vacA、cagA、cagE、iceA和babA2基因型的流行情况。

Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients.

作者信息

Chomvarin Chariya, Namwat Wises, Chaicumpar Kunyaluk, Mairiang Pisaln, Sangchan Apichat, Sripa Banchob, Tor-Udom Siripen, Vilaichone Ratha-Khon

机构信息

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

出版信息

Int J Infect Dis. 2008 Jan;12(1):30-6. doi: 10.1016/j.ijid.2007.03.012. Epub 2007 Jun 4.

DOI:10.1016/j.ijid.2007.03.012

PMID:17548220

Abstract

OBJECTIVES

To investigate the prevalence of the vacA, cagA, cagE, iceA, and babA2 genotypes in Helicobacter pylori strains isolated from Thai dyspeptic patients, and to determine whether any correlation exists between these genotypes and clinical manifestations.

METHODS

Helicobacter pylori was examined in 112 patients (62 with non-ulcer dyspepsia (gastritis), 34 with peptic ulcer disease, and 16 with gastric cancer (GCA)), detected by culture or direct detection from gastric biopsies. Allelic variants of the vacA, cagA, cagE, iceA, and babA2 genotypes were identified by using the polymerase chain reaction.

RESULTS

The positive rates for the vacAs1, vacAs2, cagA, cagE, iceA1, iceA2, and babA2 genes in H. pylori of dyspeptic patients were 100%, 0%, 98.2%, 88.4%, 45.5%, 33.1%, and 92%, respectively. The allelic variant vacAs1m1 was more prevalent (58%) than vacAs1m2 (42%). The cagA and cagE genes were commonly found together (87.5%). The most predominant genotypes were vacAs1m1, cagA, cagE, iceA1, and babA2. The various genes alone or in combination had no statistically significant association with the clinical outcomes (p>0.05).

CONCLUSION

Neither single gene nor combination of vacA, cagA, cagE, iceA, and babA2 genes was significantly helpful in predicting the clinical outcome of H. pylori infection in Thai patients. The high prevalence of these genes in H. pylori isolated from Thai patient groups suggests that H. pylori strains are geographically dependent.

摘要

目的

调查从泰国消化不良患者中分离出的幽门螺杆菌菌株中vacA、cagA、cagE、iceA和babA2基因型的流行情况，并确定这些基因型与临床表现之间是否存在相关性。

方法

对112例患者（62例非溃疡性消化不良（胃炎）、34例消化性溃疡病和16例胃癌（GCA））进行幽门螺杆菌检测，通过培养或从胃活检组织直接检测。采用聚合酶链反应鉴定vacA、cagA、cagE、iceA和babA2基因型的等位基因变体。

结果

消化不良患者幽门螺杆菌中vacAs1、vacAs2、cagA、cagE、iceA1、iceA2和babA2基因的阳性率分别为100%、0%、98.2%、88.4%、45.5%、33.1%和92%。等位基因变体vacAs1m1比vacAs1m2更常见（58%对42%）。cagA和cagE基因通常同时存在（87.5%）。最主要的基因型是vacAs1m1、cagA、cagE、iceA1和babA2。单独或组合的各种基因与临床结果均无统计学显著关联（p>0.05）。