通过腺病毒介导的促卵泡激素受体基因转导维持体外颗粒细胞功能
Maintenance of in vitro granulosa cell function by adenoviral mediated follicle stimulating hormone receptor gene transduction.
作者信息
Serikawa Takehiro, Fujita Kazuyuki, Nagata Hiroshi, Oite Takashi, Tanaka Kenichi
机构信息
Department of Obstetrics and Gynecology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
出版信息
J Assist Reprod Genet. 2006 Apr;23(4):199-206. doi: 10.1007/s10815-005-9000-6. Epub 2006 May 24.
PURPOSE
To maintain in vitro granulosa cell function by adenoviral-mediated FSHR gene transduction.
METHODS
Rat granulosa cells were cultured and transduced with adenovirus carrying FSHR gene. The number of receptors and the rate of steroidogenesis were assessed.
RESULTS
The number of FSHR on the granulosa cells was 4,874 per cell immediately after extraction, it was 2,176 by the third day, and had further reduced to 693 by the seventh day. On the third day of culture, the amount of production of estradiol by FSH stimulation also decreased to about one-quarter of the first day's quantity. Compared to the untransduced granulose cells, when the cells contained FSHR gene, the FSHR expression and steroidogenesis were both enhanced (2,176 vs. 7,206 per cell (p<0.001) and 192 vs. 5940 pg/mL (p<0.01), respectively).
CONCLUSION
Granulosa cell functions can be maintained or increased by novel gene therapy. This can be a useful component of assisted reproductive therapy.
目的
通过腺病毒介导的促卵泡激素受体（FSHR）基因转导来维持体外颗粒细胞功能。
方法
培养大鼠颗粒细胞并用携带FSHR基因的腺病毒进行转导。评估受体数量和类固醇生成率。
结果
颗粒细胞提取后即刻每个细胞的FSHR数量为4874个，第三天为2176个，到第七天进一步降至693个。培养第三天，促卵泡激素刺激产生的雌二醇量也降至第一天产量的约四分之一。与未转导的颗粒细胞相比，当细胞含有FSHR基因时，FSHR表达和类固醇生成均增强（分别为每个细胞2176个对7206个（p<0.001）和192 pg/mL对5940 pg/mL（p<0.01））。
结论
新型基因疗法可维持或增强颗粒细胞功能。这可成为辅助生殖治疗的有用组成部分。
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