采用二极管阵列检测液相色谱法对历史地图和图纸中的天然染料进行采样与鉴定。
Sampling and identification of natural dyes in historical maps and drawings by liquid chromatography with diode-array detection.
作者信息
Blanc Rosario, Espejo Teresa, López-Montes Ana, Torres David, Crovetto Guillermo, Navalón Alberto, Vílchez José Luis
机构信息
Research Group of Analytical Chemistry and Life Sciences, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, E-18071 Granada, Spain.
出版信息
J Chromatogr A. 2006 Jul 28;1122(1-2):105-13. doi: 10.1016/j.chroma.2006.04.056. Epub 2006 Jun 6.
A simple and rapid liquid chromatographic with diode-array UV-vis spectrophotometric detection (HPLC-DAD) method for identification of natural dyes has been developed. Chromatographic retention of carminic acid, indigotin, crocetin, gambogic acid, alizarin and purpurin has been studied. The mobile phase consisted of 40 mM SDS-10 mM phosphate buffer solution (pH 2.3)-0.1% TFA (eluent A) and acetonitrile (eluent B) using a programmed gradient (5% B to 95% B). Analyses were carried out on a Phenomenex, Luna 5u NH2 100(a) column (250 mm x 4.60 mm i.d., 5 microm particle) and the operating conditions were: 0.6 ml min(-1) flow rate, 20 microl volume injection and 35 degrees C column temperature. Extracts of samples of natural dyes taken from historical maps belonging to The Royal Chancellery Archives in Granada were successfully analyzed using the proposed method including a new technique for sampling.
已开发出一种用于鉴定天然染料的简单快速的液相色谱-二极管阵列紫外-可见分光光度检测法（HPLC-DAD）。研究了胭脂红酸、靛蓝、藏红花素、藤黄酸、茜素和紫红素的色谱保留情况。流动相由40 mM SDS-10 mM磷酸盐缓冲溶液（pH 2.3）-0.1% TFA（洗脱液A）和乙腈（洗脱液B）组成，采用程序梯度（5% B至95% B）。分析在Phenomenex Luna 5u NH2 100(a)柱（250 mm×4.60 mm内径，5微米粒径）上进行，操作条件为：流速0.6 ml min(-1)，进样体积20微升，柱温为35℃。使用所提出的方法，包括一种新的采样技术，成功分析了从格拉纳达皇家总理府档案馆历史地图中获取的天然染料样品提取物。
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