Orska-Gawryś Jowita, Surowiec Izabella, Kehl Jerzy, Rejniak Hanna, Urbaniak-Walczak Katarzyna, Trojanowicz Marek
Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland.
J Chromatogr A. 2003 Mar 14;989(2):239-48. doi: 10.1016/s0021-9673(03)00083-9.
Reversed-phase HPLC with diode-array UV-Vis spectrophotometric detection has been used for identification of natural dyes in extracts from wool and silk fibres from archeological textiles. The examined objects originate from 4th to 12th Century Egypt and belong to the collection of Early Christian Art of the National Museum in Warsaw. Extraction from fibres was carried out with HCl solution containing ethanol or with warm pyridine. As the main individual chemical components of natural dyes, anthraquinone, indigoid and flavonoid dyes including alizarin, purpurin, luteolin, apigenin, carminic acid, ellagic acid, gallic acid, laccaic acids A and B and indigotin were found. For pyridine extracts another mobile phase with an optimized gradient of organic modifier concentration was used. With such an eluent the appearance of double peaks for indigotin and indirubin was eliminated. For acidic extraction of dyes from fibres, ethanol was used. Due to its higher boiling point than methanol it evaporates slower from the extraction solution enabling a more efficient extraction of dyes.
采用反相高效液相色谱法结合二极管阵列紫外 - 可见分光光度检测法，对考古纺织品中羊毛和丝绸纤维提取物中的天然染料进行鉴定。所检测的物品源自公元4世纪至12世纪的埃及，属于华沙国家博物馆早期基督教艺术藏品。纤维提取采用含乙醇的盐酸溶液或温热吡啶进行。作为天然染料的主要化学成分，发现了蒽醌、靛类和黄酮类染料，包括茜素、紫茜素、木犀草素、芹菜素、胭脂红酸、鞣花酸、没食子酸、紫胶酸A和B以及靛蓝。对于吡啶提取物，使用了有机改性剂浓度优化梯度的另一种流动相。使用这种洗脱剂消除了靛蓝和靛玉红的双峰现象。从纤维中酸性提取染料时使用的是乙醇。由于其沸点高于甲醇，它从提取溶液中蒸发得较慢，从而能够更有效地提取染料。
