Daniel Roy M, Danson Michael J, Eisenthal Robert, Lee Charles K, Peterson Michelle E
Department of Biological Sciences, University of Waikato, Private Bag 3105, Hamilton 3240, New Zealand.
Extremophiles. 2008 Jan;12(1):51-9. doi: 10.1007/s00792-007-0089-7. Epub 2007 Sep 13.
The two established thermal properties of enzymes are their activation energy and their thermal stability. Arising from careful measurements of the thermal behaviour of enzymes, a new model, the Equilibrium Model, has been developed to explain more fully the effects of temperature on enzymes. The model describes the effect of temperature on enzyme activity in terms of a rapidly reversible active-inactive transition, in addition to an irreversible thermal inactivation. Two new thermal parameters, Teq and Delta Heq, describe the active-inactive transition, and enable a complete description of the effect of temperature on enzyme activity. We review here the Model itself, methods for the determination of Teq and Delta Heq, and the implications of the Model for the environmental adaptation and evolution of enzymes, and for biotechnology.
酶已确定的两个热性质是它们的活化能和热稳定性。通过对酶热行为的仔细测量，已开发出一种新模型——平衡模型，以更全面地解释温度对酶的影响。该模型除了描述不可逆的热失活外，还从快速可逆的活性-非活性转变方面描述了温度对酶活性的影响。两个新的热参数Teq和ΔHeq描述了活性-非活性转变，并能完整描述温度对酶活性的影响。在此，我们综述该模型本身、Teq和ΔHeq的测定方法，以及该模型对酶的环境适应性和进化以及生物技术的意义。
