Llamas Susana, Moreno Ignacio M, García-Arenal Fernando
Departamento de Biotecnología, ETSI Agrónomos, Universidad Politécnica de Madrid, 28040 Madrid, Spain.
J Gen Virol. 2006 Jul;87(Pt 7):2085-2088. doi: 10.1099/vir.0.81871-0.
Coat-protein (CP) hybrids between Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV) were engineered to analyse reported CP-associated differences between these viruses. CP portions delimited by aa 1-59, 60-148 and 149-219 were exchanged in all possible combinations within TAV RNA3. The seven possible chimeras were able to replicate in tobacco protoplasts to similar levels, but only those having residues 1-59 or 60-148 from CMV were infectious to tobacco plants, a common host for CMV and TAV, and formed stable particles. When most of the movement protein (MP) of TAV was substituted for that of CMV, infectivity of CP hybrids did not vary. No hybrid was able to infect cucumber plants, a host for CMV and not for TAV. Need for MP-CP compatibility could explain these results, but shows that MP-CP compatibility conditions the use of CP chimeras to map CP-associated differences between CMV and TAV.
构建了黄瓜花叶病毒（CMV）和番茄不孕病毒（TAV）之间的外壳蛋白（CP）杂种，以分析报道的这两种病毒之间与CP相关的差异。在TAV RNA3内，由氨基酸1-59、60-148和149-219界定的CP部分以所有可能的组合进行了交换。这七种可能的嵌合体能够在烟草原生质体中以相似水平复制，但只有那些具有来自CMV的1-59或60-148残基的嵌合体对烟草植株具有感染性，烟草是CMV和TAV的共同寄主，并且形成了稳定的颗粒。当TAV的大部分运动蛋白（MP）被CMV的运动蛋白替代时，CP杂种的感染性没有变化。没有杂种能够感染黄瓜植株，黄瓜是CMV的寄主而不是TAV的寄主。对MP-CP兼容性的需求可以解释这些结果，但表明MP-CP兼容性决定了使用CP嵌合体来定位CMV和TAV之间与CP相关的差异。
